The Synergistic Anticancer Effect of Dual Drug- (CisplatinEpigallocatechin Gallate) Loaded Gelatin Nanoparticles for Lung Cancer Treatment
Joint Authors
Tseng, Ching-Li
Chen, Yin-Ju
Wang, Zhi-Weng
Lu, Tung-Ling
Gomez, Clinton B.
Fang, Hsu-Wei
Wei, Yang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-05
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Lung cancer has the highest mortality of any cancer worldwide, and cisplatin is a first-line chemotherapeutic agent for lung cancer treatment.
Unfortunately, cisplatin resistance is a common cause of therapeutic failure.
The ability to overcome chemoresistance is crucial to the effective treatment of lung cancer.
Recently, epigallocatechin gallate (EGCG), a type of polyphenol extracted from tea, has been shown to suppress the rapid proliferation of cancer cells, including lung cancer.
We tested whether nanoparticles (NPs) carrying a dual drug load, cisplatin and EGCG, could overcome chemoresistance to cisplatin, by working together to kill lung cancer cells.
Self-assembling gelatin/EGCG nanoparticles (GE) were synthesized, and cisplatin was then incorporated, to construct a dual drug nanomedicine (EGCG/cisplatin-loaded gelatin nanoparticle, named as GE-Pt NP).
The particle size and zeta potential were examined by dynamic light scattering (DLS).
The morphological structure of GE-Pt NPs was observed by transmission electron microscopy (TEM).
In vitro testing was performed using a human lung adenocarcinoma cell line (A549).
A cytotoxicity examination was performed, using a WST-8 cell proliferation assay.
Intracellular cisplatin content was quantified by inductively coupled plasma mass spectrometry (ICP-MS).
In conclusion, we successfully prepared GE-Pt NPs, as spherical structures, approximately 75 nm in diameter, with a positive charge (+19.83±0.25 mV).
The encapsulation rate of cisplatin in GE-Pt was about 63.7%, and the EGCG loading rate was around 89%.
A relatively low concentration of GE-Pt NPs (EGCG 5 μg/mL : cisplatin 2 μg/mL) exhibited significant cytotoxicity, compared to cisplatin alone.
The GE-Pt NPs are freely taken up by cells via endocytosis, raising the intracellular cisplatin concentration to a therapeutic level.
We consider that combination therapy of cisplatin and EGCG in nanoparticles (GE-Pt NPs) may help overcome cisplatin resistance and could effectively be used in the treatment of lung cancer.
American Psychological Association (APA)
Chen, Yin-Ju& Wang, Zhi-Weng& Lu, Tung-Ling& Gomez, Clinton B.& Fang, Hsu-Wei& Wei, Yang…[et al.]. 2020. The Synergistic Anticancer Effect of Dual Drug- (CisplatinEpigallocatechin Gallate) Loaded Gelatin Nanoparticles for Lung Cancer Treatment. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1188618
Modern Language Association (MLA)
Chen, Yin-Ju…[et al.]. The Synergistic Anticancer Effect of Dual Drug- (CisplatinEpigallocatechin Gallate) Loaded Gelatin Nanoparticles for Lung Cancer Treatment. Journal of Nanomaterials No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1188618
American Medical Association (AMA)
Chen, Yin-Ju& Wang, Zhi-Weng& Lu, Tung-Ling& Gomez, Clinton B.& Fang, Hsu-Wei& Wei, Yang…[et al.]. The Synergistic Anticancer Effect of Dual Drug- (CisplatinEpigallocatechin Gallate) Loaded Gelatin Nanoparticles for Lung Cancer Treatment. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1188618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188618