Jurkat T Cell Detectability and Toxicity Evaluation of Low-Temperature Synthesized Cadmium Quantum Dots
Joint Authors
Vo, N. T.
Lam, Q. V.
Vu, Thi Bich
Diep, Hong Tran Thi
Le Khanh, Thien
Van, Hieu Tran
Nguyen, Thanh Binh
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Early and highly accurate detection of diverse diseases is in urgent demand than ever, especially for cancers and infectious ones.
Among possibilities, biosensing by utilizing conjugated nanoparticles is still a method of choice.
However, the toxicity of quantum dots remains a big matter of concern in those biooriented applications.
In this study, mercaptosuccinic acid-coated cadmium selenide quantum dots of approximately 2.3 nm were synthesized with a simple green method at low temperature and cost-saving chemicals.
The influence of synthesis factors was investigated with different spectroscopic methods.
The toxicity issue was evaluated on the NIH-3T3 cell line (ATCC® CRL-1658™) and an MTT assay, revealing a secure threshold of 20 μg/ml.
Consequently, successful conjugation to the CD3 antibody including an A/G protein bridge was implemented and verified with fluorescent methods.
Finally, Jurkat T cell detectability of conjugated CdSe was successfully validated with fluorescent microscopy.
The CdSe-based products are accessible for future biosensing applications.
American Psychological Association (APA)
Vo, N. T.& Vu, Thi Bich& Diep, Hong Tran Thi& Le Khanh, Thien& Van, Hieu Tran& Nguyen, Thanh Binh…[et al.]. 2020. Jurkat T Cell Detectability and Toxicity Evaluation of Low-Temperature Synthesized Cadmium Quantum Dots. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1188623
Modern Language Association (MLA)
Vo, N. T.…[et al.]. Jurkat T Cell Detectability and Toxicity Evaluation of Low-Temperature Synthesized Cadmium Quantum Dots. Journal of Nanomaterials No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1188623
American Medical Association (AMA)
Vo, N. T.& Vu, Thi Bich& Diep, Hong Tran Thi& Le Khanh, Thien& Van, Hieu Tran& Nguyen, Thanh Binh…[et al.]. Jurkat T Cell Detectability and Toxicity Evaluation of Low-Temperature Synthesized Cadmium Quantum Dots. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1188623
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188623