Codelivery of Emodin and Diammonium Glycyrrhizinate by Anti-alpha8 Integrin-Conjugated Immunoliposomes for the Treatment of Renal Fibrosis
Joint Authors
Sun, Jiang
Yao, Wenjie
Luo, Xiaoting
Xu, Zhishi
Wei, Yinghui
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-28
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The targeted delivery of therapeutics to the kidneys has a profound potential for the management of renal fibrosis.
Thus, we developed a drug delivery system that targets mesangial cells by conjugating anti-alpha8 integrin to the surface of liposomes.
We coloaded emodin (EMO) and diammonium glycyrrhizinate (DAG) to the immunoliposomes for combined therapy.
The coloaded immunoliposomes were small size (92.4±0.4 nm), narrowly distributed, and with nearly neutral zeta potential and good stability.
The encapsulation rate of EMO and DAG in immunoliposomes was 45.5±2.0% and 44.3±1.1%, respectively.
Using a BCA assay, the actual number of antibody molecules attached to a single liposome was determined as being approximately 41.
An in vitro release study showed that EMO and DAG could be ratiometrically released from the immunoliposomes, which means that an optimized synergistic ratio of the two drugs could be achieved.
Studies on cellular uptake studies demonstrated an approximately 3-fold increase for immunoliposomes in HBZY-1 cells compared to nonconjugated liposomes.
In vitro cell growth inhibition and Western Blot assay revealed that the coloaded immunoliposomes exhibited a stronger and synergistic in vitro antifibrosis effect against NIH3T3 and HBZY-1 cells in vitro.
Taken together, it indicated that anti-alpha8 integrin-modified immunoliposomes for codelivery of EMO and DAG have great potential for targeting the kidneys for the treatment of renal fibrosis.
American Psychological Association (APA)
Sun, Jiang& Yao, Wenjie& Luo, Xiaoting& Xu, Zhishi& Wei, Yinghui. 2020. Codelivery of Emodin and Diammonium Glycyrrhizinate by Anti-alpha8 Integrin-Conjugated Immunoliposomes for the Treatment of Renal Fibrosis. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1188627
Modern Language Association (MLA)
Sun, Jiang…[et al.]. Codelivery of Emodin and Diammonium Glycyrrhizinate by Anti-alpha8 Integrin-Conjugated Immunoliposomes for the Treatment of Renal Fibrosis. Journal of Nanomaterials No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1188627
American Medical Association (AMA)
Sun, Jiang& Yao, Wenjie& Luo, Xiaoting& Xu, Zhishi& Wei, Yinghui. Codelivery of Emodin and Diammonium Glycyrrhizinate by Anti-alpha8 Integrin-Conjugated Immunoliposomes for the Treatment of Renal Fibrosis. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1188627
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188627