Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2
Joint Authors
Wang, Chang
Ren, Qiong-Qiong
Yang, Fen
Ren, Wu
Jiang, Wen-Shuai
Zhao, Zong-Ya
Chen, Jun
Lu, Xuan-Yi
Yu, Yi
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
An enzyme-based amperometric biosensor was fabricated for detecting hydrogen peroxide (H2O2).
Horseradish peroxidase (HRP) was modified using functionalized fluorescent gold nanoclusters (AuNCs) via biomineralization.
HRP-AuNCs were successfully immobilized on multiwalled carbon nanotube- (MWCNT-) coated carbon fiber ultramicroelectrodes (CFUMEs).
The AuNCs, which act as molecular electric wires, effectively promote the electron transfer between the enzyme active center and the electrode.
Additionally, the HRP conjugated with the AuNCs retains its biological activity, which enables the catalytic reaction of H2O2.
The HRP-AuNCs/MWCNTs/CFUMEs have been proven as excellent amperometric sensors for H2O2.
The sensitivity of the H2O2 biosensor is 3.0×10−4 A/M, and the detection limit is estimated to be 443 nM.
Furthermore, the biosensor exhibited long-term stability and good reproducibility.
Moreover, the biosensor has been tested by determining the H2O2 concentration in calf serum samples.
American Psychological Association (APA)
Ren, Qiong-Qiong& Yang, Fen& Ren, Wu& Wang, Chang& Jiang, Wen-Shuai& Zhao, Zong-Ya…[et al.]. 2020. Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1188632
Modern Language Association (MLA)
Ren, Qiong-Qiong…[et al.]. Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2. Journal of Nanomaterials No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1188632
American Medical Association (AMA)
Ren, Qiong-Qiong& Yang, Fen& Ren, Wu& Wang, Chang& Jiang, Wen-Shuai& Zhao, Zong-Ya…[et al.]. Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1188632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188632