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Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase and NF-κB Activation
Joint Authors
Kim, Seung-Hyung
Kwak, Yi-Seong
Endale, Mehari
Kim, Tae-Hwan
Yun, Bong-Sik
Kim, Na-Mi
Cho, Jae Youl
Rhee, Man Hee
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Torilin, a sesquiterpene isolated from the fruits of Torilis japonica, has shown antimicrobial, anticancer, and anti-inflammatory properties.
However, data on the mechanism of torilin action against inflammation is limited.
This study aimed at determining the anti-inflammatory property of torilin in LPS-induced inflammation using in vitro model of inflammation.
We examined torilin’s effect on expression levels of inflammatory mediators and cytokines in LPS-stimulated RAW 264.7 macrophages.
The involvement of NF-kB and AP-1, MAP kinases, and adaptor proteins were assessed.
Torilin strongly inhibited LPS-induced NO release, iNOS, PGE2, COX-2, NF-α, IL-1β, IL-6, and GM-CSF gene and protein expressions.
In addition, MAPKs were also suppressed by torilin pretreatment.
Involvement of ERK1/2, P38MAPK, and JNK1/2 was further confirmed by PD98059, SB203580, and SP600125 mediated suppression of iNOS and COX-2 proteins.
Furthermore, torilin attenuated NF-kB and AP-1 translocation, DNA binding, and reporter gene transcription.
Interestingly, torilin inhibited TAK1 kinase activation with the subsequent suppression of MAPK-mediated JNK, p38, ERK1/2, and AP-1 (ATF-2 and c-jun) activation and IKK-mediated I-κBα degradation, p65/p50 activation, and translocation.
Together, the results revealed the suppression of NF-κB and AP-1 regulated inflammatory mediator and cytokine expressions, suggesting the test compound’s potential as a candidate anti-inflammatory agent.
American Psychological Association (APA)
Endale, Mehari& Kim, Tae-Hwan& Kwak, Yi-Seong& Kim, Na-Mi& Kim, Seung-Hyung& Cho, Jae Youl…[et al.]. 2017. Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase and NF-κB Activation. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188643
Modern Language Association (MLA)
Endale, Mehari…[et al.]. Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase and NF-κB Activation. Mediators of Inflammation No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1188643
American Medical Association (AMA)
Endale, Mehari& Kim, Tae-Hwan& Kwak, Yi-Seong& Kim, Na-Mi& Kim, Seung-Hyung& Cho, Jae Youl…[et al.]. Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase and NF-κB Activation. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188643
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188643