Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of CEBP-Alpha in Mature 3T3-L1 Adipocytes
Joint Authors
Sergio, Cuellar-Rufino
Rossana Citlali, Zepeda
Mónica, Flores-Muñoz
Isela, Santiago-Roque
Omar, Arroyo-Helguera
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Overweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health.
This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes.
The mature adipocytes were treated with doses from 1 to 100 µm of lugol for 0.5, 6, and 24 h.
The results showed that mature adipocytes exposed to lugol decrease their viability and increase caspase-3 activity with a lethal dose (LD50) of 473 µm.
In mature adipocytes, lugol decreased the total intracellular lipid content, being significant at doses of 10 and 100 µm after 6 and 24 h of treatment (P<0.01), and the accumulation of intracellular triglycerides decreased after 24 h of exposure to lugol (P<0.05).
Lugol treatment significantly increases the release of glycerol to the culture medium (P<0.05).
The levels of adipocyte-specific transcription factors C/EBP-α were downregulated and PPAR-γ upregulated after 30 min with lugol.
These results indicate a lipolytic effect of lugol dependent on PPAR-γ and C/EBP-α expression in mature 3T3-L1 adipocytes.
American Psychological Association (APA)
Sergio, Cuellar-Rufino& Rossana Citlali, Zepeda& Mónica, Flores-Muñoz& Isela, Santiago-Roque& Omar, Arroyo-Helguera. 2020. Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of CEBP-Alpha in Mature 3T3-L1 Adipocytes. Journal of Nutrition and Metabolism،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1188661
Modern Language Association (MLA)
Sergio, Cuellar-Rufino…[et al.]. Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of CEBP-Alpha in Mature 3T3-L1 Adipocytes. Journal of Nutrition and Metabolism No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1188661
American Medical Association (AMA)
Sergio, Cuellar-Rufino& Rossana Citlali, Zepeda& Mónica, Flores-Muñoz& Isela, Santiago-Roque& Omar, Arroyo-Helguera. Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of CEBP-Alpha in Mature 3T3-L1 Adipocytes. Journal of Nutrition and Metabolism. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1188661
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188661