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UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1−− Microglia In Vitro and In Vivo
Joint Authors
Heo, Woon
Kim, Joo Young
Lim, Hye Min
Han, Jung Woo
Baba, Yoshihiro
Kurosaki, Tomohiro
Lee, Min Goo
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
STIM1 is the only currently known intracellular calcium sensor that functions as the calcium influx regulator controlling immune cell activation.
STIM1 function in immune cell calcium signalling has been studied extensively; however, its role in microglia, innate immune cells in brain, has not been fully understood.
Here, we report that STIM1−/− murine microglia lost store-operated calcium influx and displayed aberrant immunological functions.
Microglial functions regulated by chronic and global Ca2+i changes were reduced significantly, including cytokine releases and opsonin-dependent phagocytosis.
More dramatically, cellular functions governed by Ca2+ regulation in local microdomains at the cell periphery, such as UDP-induced phagocytosis and ATP-stimulated chemotactic migration, were severely reduced in STIM1−/− microglia.
Interestingly, UDP-induced Orai1 mobilization to the peripheral region was greatly attenuated in STIM1−/− microglia.
Their chemotactic migration defect was reproduced in vivo in embryonic brain; the aggregated number of STIM1−/− microglia in LPS- (lipopolysaccharide-) injected lesions was much smaller than that in wild-type microglia.
Furthermore, the neuron phagoptosis activities of activated microglia were significantly diminished in the STIM1−/− microglia.
These in vitro and in vivo results suggest that STIM1-mediated store-operated calcium entry is important for the regulation of global Ca2+i changes which differentiates into active immune state of microglia, but it is more crucial for the regulation of local [Ca2+] microdomains which mediates the acute motility of murine microglia.
American Psychological Association (APA)
Lim, Hye Min& Heo, Woon& Han, Jung Woo& Baba, Yoshihiro& Kurosaki, Tomohiro& Lee, Min Goo…[et al.]. 2017. UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1−− Microglia In Vitro and In Vivo. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188742
Modern Language Association (MLA)
Lim, Hye Min…[et al.]. UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1−− Microglia In Vitro and In Vivo. Mediators of Inflammation No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1188742
American Medical Association (AMA)
Lim, Hye Min& Heo, Woon& Han, Jung Woo& Baba, Yoshihiro& Kurosaki, Tomohiro& Lee, Min Goo…[et al.]. UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1−− Microglia In Vitro and In Vivo. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188742
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188742