Role of 17β-Estradiol on Cell Proliferation and Mitochondrial Fitness in Glioblastoma Cells
Joint Authors
Li Volti, Giovanni
Tibullo, Daniele
Castracani, Carlo Castruccio
Longhitano, Lucia
Distefano, Alfio
Anfuso, Daniela
Kalampoka, Stavroula
La Spina, Enrico
Astuto, Marinella
Avola, Roberto
Caruso, Massimo
Nicolosi, Daria
Giallongo, Cesarina
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Gliomas are the most common primary tumors of the central nervous system (CNS) in the adult.
Previous data showed that estrogen affects cancer cells, but its effect is cell-type-dependent and controversial.
The present study aimed to analyze the effects of estradiol (E2, 5 nM) in human glioblastoma multiforme U87-MG cells and how it may impact on cell proliferation and mitochondrial fitness.
We monitored cell proliferation by xCELLigence technology and mitochondrial fitness by assessing the expression of genes involved in mitochondrial biogenesis (PGC1α, SIRT1, and TFAM), oxidative phosphorylation (ND4, Cytb, COX-II, COX IV, NDUFA6, and ATP synthase), and dynamics (OPA1, MNF2, MNF1, and FIS1).
Finally, we evaluated Nrf2 nuclear translocation by immunocytochemical analysis.
Our results showed that E2 resulted in a significant increase in cell proliferation, with a significant increase in the expression of genes involved in various mechanisms of mitochondrial fitness.
Finally, E2 treatment resulted in a significant increase of Nrf2 nuclear translocation with a significant increase in the expression of one of its target genes (i.e., heme oxygenase-1).
Our results suggest that E2 promotes proliferation in glioblastoma cells and regulate the expression of genes involved in mitochondrial fitness and chemoresistance pathway.
American Psychological Association (APA)
Castracani, Carlo Castruccio& Longhitano, Lucia& Distefano, Alfio& Anfuso, Daniela& Kalampoka, Stavroula& La Spina, Enrico…[et al.]. 2020. Role of 17β-Estradiol on Cell Proliferation and Mitochondrial Fitness in Glioblastoma Cells. Journal of Oncology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1188870
Modern Language Association (MLA)
Castracani, Carlo Castruccio…[et al.]. Role of 17β-Estradiol on Cell Proliferation and Mitochondrial Fitness in Glioblastoma Cells. Journal of Oncology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1188870
American Medical Association (AMA)
Castracani, Carlo Castruccio& Longhitano, Lucia& Distefano, Alfio& Anfuso, Daniela& Kalampoka, Stavroula& La Spina, Enrico…[et al.]. Role of 17β-Estradiol on Cell Proliferation and Mitochondrial Fitness in Glioblastoma Cells. Journal of Oncology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1188870
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188870