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Circulating Tumor DNA Using Tagged Targeted Deep Sequencing to Assess Minimal Residual Disease in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy
Joint Authors
Garlaschi, Alessandro
Cirmena, Gabriella
Garuti, Anna
De Mariano, Marilena
Coco, Simona
Ferrando, Lorenzo
Isnaldi, Edoardo
Barbero, Valentina
Fregatti, Piero
Del Mastro, Lucia
Ferrando, Fabio
Gonella, Roberta
Friedman, Daniele
Ballestrero, Alberto
Zoppoli, Gabriele
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In breast cancer patients undergoing neoadjuvant chemotherapy before surgery, there is an unmet need for noninvasive predictive biomarkers of response.
The analysis of circulating tumor DNA (ctDNA) in particular has been the object of several reports, but few of them have studied the applicability of tagged targeted deep sequencing (tTDS) to clinical practice and its performance compared with droplet digital PCR (ddPCR).
Here, we present the first results from an ongoing study involving a prospectively accrued, monocentric cohort of patients affected by invasive breast cancer, undergoing neoadjuvant chemotherapy followed by surgery with curative intent as per clinical practice.
A pretreatment tumor biopsy and plasma samples were collected before and during treatment, after surgery, and every six months henceforth or until relapse, whichever came first.
Pretreatment biopsies were sequenced with a 409-gene massive parallel sequencing (MPS) panel, allowing the identification of target mutations and their research in plasma by tTDS and ddPCR as a complementary approach.
Using tTDS, we demonstrated the presence of at least one deleterious mutation in all the relapsed cases we studied (n = 4), with an average lead time of six months before clinical relapse.
The association with ddPCR was suboptimal, and only one relapsed patient could be identified with such method.
tTDS shows potential as an early noninvasive method for the detection of MRD in BC patients.
American Psychological Association (APA)
Cirmena, Gabriella& Garuti, Anna& De Mariano, Marilena& Coco, Simona& Ferrando, Lorenzo& Isnaldi, Edoardo…[et al.]. 2020. Circulating Tumor DNA Using Tagged Targeted Deep Sequencing to Assess Minimal Residual Disease in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy. Journal of Oncology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1189100
Modern Language Association (MLA)
Cirmena, Gabriella…[et al.]. Circulating Tumor DNA Using Tagged Targeted Deep Sequencing to Assess Minimal Residual Disease in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy. Journal of Oncology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1189100
American Medical Association (AMA)
Cirmena, Gabriella& Garuti, Anna& De Mariano, Marilena& Coco, Simona& Ferrando, Lorenzo& Isnaldi, Edoardo…[et al.]. Circulating Tumor DNA Using Tagged Targeted Deep Sequencing to Assess Minimal Residual Disease in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy. Journal of Oncology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1189100
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1189100