SPARC Levels Modulate the Capacity of Mitomycin to Inhibit the Proliferation of Human Tenon’s Capsule Fibroblasts
Joint Authors
Ni, Shouxiang
Zhou, Weiyan
Guo, Yuanyuan
Hou, Jiangping
Shen, Jiaquan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
To evaluate the role of SPARC in the antiproliferation effect of MMC on human Tenon’s fibroblasts (HTF).
Method.
Sixteen PACG patients aged 59 ± 10 years (31–72 years), including 6 males and 10 females, were recruited.
Tenon tissue was harvested during filtering surgery.
Cell density was evaluated after MMC application with different concentrations and application times, by which the optimized MMC application modality was determined.
MMC, si-SPARC, or SPARC protein was used when needed to evaluate the cell densities under different conditions, by which the role of SPARC in MMC-mediated antifibrotic process was identified.
Results.
Considering that the cell densities, as well as SPARC expression on mRNA and protein levels, are relatively stable when the MMC concentration is higher than 0.02% and exposure time longer than 90 s, we chose the MMC application pattern with 0.02% and 90 s as an optimized pattern for the downstream work.
Compared to control, the si-SPARC and MMC downregulated the SPARC protein by 91% (P<0.01) and 65% (P<0.01) and mRNA by 96% (P<0.01) and 64% (P<0.01), respectively.
MMC decreases the cell densities by 53.50% compared to control.
si-SPARC + MMC dramatically deceased the cell density no matter compared to the control group (P<0.01) or MMC group (P<0.01); correspondingly, the relative collagen gel area in the MMC + si-SPARC group was higher than that in the MMC group or si-SPARC group (P<0.05).
The reactive oxygen species expression in the MMC + si-SPARC group is higher than that in the MMC group (P<0.05).
Conclusion.
This study demonstrates that in HTF, (1) MMC downregulates the expression of SPARC in protein and mRNA levels; (2) SPARC depletion has synergistic effect on the antifibrotic effect of MMC; and (3) reactive oxygen species are the possible mediator in the antifibrotic effect of MMC and si-SPARC.
American Psychological Association (APA)
Guo, Yuanyuan& Ni, Shouxiang& Zhou, Weiyan& Hou, Jiangping& Shen, Jiaquan. 2020. SPARC Levels Modulate the Capacity of Mitomycin to Inhibit the Proliferation of Human Tenon’s Capsule Fibroblasts. Journal of Ophthalmology،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1189490
Modern Language Association (MLA)
Guo, Yuanyuan…[et al.]. SPARC Levels Modulate the Capacity of Mitomycin to Inhibit the Proliferation of Human Tenon’s Capsule Fibroblasts. Journal of Ophthalmology No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1189490
American Medical Association (AMA)
Guo, Yuanyuan& Ni, Shouxiang& Zhou, Weiyan& Hou, Jiangping& Shen, Jiaquan. SPARC Levels Modulate the Capacity of Mitomycin to Inhibit the Proliferation of Human Tenon’s Capsule Fibroblasts. Journal of Ophthalmology. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1189490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1189490