Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride
Joint Authors
Carracedo, Gonzalo
Carpena-Torres, Carlos
Pintor, Jesús
Pérez de Lara, María Jesús
Huete-Toral, Fernando
Crooke, Almudena
Pastrana, Cristina
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%.
Materials and Methods.
An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10).
Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily.
Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment.
Results.
After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group.
Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001.
Conclusions.
The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).
American Psychological Association (APA)
Carpena-Torres, Carlos& Pintor, Jesús& Pérez de Lara, María Jesús& Huete-Toral, Fernando& Crooke, Almudena& Pastrana, Cristina…[et al.]. 2020. Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride. Journal of Ophthalmology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1189632
Modern Language Association (MLA)
Carpena-Torres, Carlos…[et al.]. Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride. Journal of Ophthalmology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1189632
American Medical Association (AMA)
Carpena-Torres, Carlos& Pintor, Jesús& Pérez de Lara, María Jesús& Huete-Toral, Fernando& Crooke, Almudena& Pastrana, Cristina…[et al.]. Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride. Journal of Ophthalmology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1189632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1189632