Occurrence, Morphometric, and Molecular Investigation of Cattle and Buffalo Liver Adult Fluke in Peninsular Malaysia Main Abattoirs
Joint Authors
Diyana, J. Nur Alia
Lokman, I. H.
Fazila, S. H. Nur
Ibitoye, E. B.
Chandrawathani, P.
Mahiza, M. I. Nur
Latiffah, H.
Hazfalinda, H. Noor
Juriah, K.
Source
Journal of Parasitology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Fascioliasis is a parasitic disease of human and animal caused by Fasciola gigantica (F.
gigantica) and Fasciola hepatica (F.
hepatica).
More than 700 million of grazing animals and over 180 million human population are at the risk of fascioliasis.
This study was conducted in Banting, Ipoh, Shah Alam, and Taiping abattoirs within Peninsular Malaysia to determine the occurrence and identify the species of liver flukes, causing liver condemnation in cattle and buffaloes.
Within a study period from January to December 2018, a total of 25 condemned bovine livers were collected from Banting, Ipoh, Shah Alam, and Taiping abattoirs and analyzed.
Taiping abattoir had the highest occurrence of fascioliasis [14/1014 (1.38%)], and Shah Alam abattoir had the least occurrence [1/3377 (0.03%)].
From all the sampled livers, the average number of adult flukes recorded ranged from 1 to 83.
A total of 440 adult flukes were studied morphometrically, using parameters such as body length (BL), body width (BW), cone length (CL), cone width (CL), body area (BA), body perimeter (BP), the distance between the ventral sucker and posterior end of body (VS-P), BL/BW ratio, BL/V-SP, and body roundness (BR).
Furthermore, molecular analysis was conducted using PCR-RFLP methods to distinguish between F.
gigantica and F.
hepatica involving ITS1 primer and RsaI restriction enzyme.
RFLP pattern with RsaI produced a consistent pattern of 360,100 and 60 bp fragments in F.
hepatica, whereas F.
gigantica worms had a profile of 360,170, and 60 bp in size.
The morphometric and molecular analysis results indicated that cattle and buffaloes slaughtered at Banting, Shah Alam, Taiping, and Ipoh abattoirs were infected with F.
gigantica.
American Psychological Association (APA)
Diyana, J. Nur Alia& Mahiza, M. I. Nur& Latiffah, H.& Fazila, S. H. Nur& Lokman, I. H.& Hazfalinda, H. Noor…[et al.]. 2020. Occurrence, Morphometric, and Molecular Investigation of Cattle and Buffalo Liver Adult Fluke in Peninsular Malaysia Main Abattoirs. Journal of Parasitology Research،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1190085
Modern Language Association (MLA)
Diyana, J. Nur Alia…[et al.]. Occurrence, Morphometric, and Molecular Investigation of Cattle and Buffalo Liver Adult Fluke in Peninsular Malaysia Main Abattoirs. Journal of Parasitology Research No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1190085
American Medical Association (AMA)
Diyana, J. Nur Alia& Mahiza, M. I. Nur& Latiffah, H.& Fazila, S. H. Nur& Lokman, I. H.& Hazfalinda, H. Noor…[et al.]. Occurrence, Morphometric, and Molecular Investigation of Cattle and Buffalo Liver Adult Fluke in Peninsular Malaysia Main Abattoirs. Journal of Parasitology Research. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1190085
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1190085