Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line
Joint Authors
Abdel-Halim, Khaled Y.
Osman, Safaa R.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-08
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Insecticide imidacloprid and herbicide glyphosate have a broad spectrum of applicable use in the agricultural sector of Egypt.
Their ability to induce in vitro cytotoxic and oxidative stress on normal human cells (prostate epithelial WPM-Y.1 cell line) was evaluated with the methyl tetrazolium test (MTT) and histopathological investigation.
Cell viability was evaluated with an MTT test for 24 h.
The median inhibition concentration (IC50) values were 0.023 and 0.025 mM for imidacloprid and glyphosate, respectively.
Sublethal concentrations: 1/10 and 1/50 of IC50 and IC50 levels significantly induced an increase in the lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) level compared with the untreated cells.
Rapid decrease in the glutathione (GSH) content and glutathione-S-transferase (GST) activity was induced.
Significant increases were recorded in activities of catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), respectively, compared with the control group.
Transmission electron microscopic (TEM) investigation showed significant defects in the cells following pesticide treatments for 24 h.
Therefore, it is concluded that imidacloprid and glyphosate are very toxic in vitro assays and able to induce apoptotic effects as well as oxidative stress.
So, these findings provide a scenario of multibiomarkers to achieve the imposed risks of pesticides at low doses.
American Psychological Association (APA)
Abdel-Halim, Khaled Y.& Osman, Safaa R.. 2020. Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line. Journal of Toxicology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1190897
Modern Language Association (MLA)
Abdel-Halim, Khaled Y.& Osman, Safaa R.. Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line. Journal of Toxicology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1190897
American Medical Association (AMA)
Abdel-Halim, Khaled Y.& Osman, Safaa R.. Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line. Journal of Toxicology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1190897
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1190897