IL-33 Inhibits Hepatitis B Virus through Its Receptor ST2 in Hydrodynamic HBV Mouse Model
Joint Authors
Wu, Ruihong
Niu, Jun-Qi
Xu, Damo
Li, Dong
Gao, Xiuzhu
Xu, Hongqin
Zhan, MengRu
Ding, Yanhua
Wang, Xiaomei
Chi, Xiumei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Interleukin-33 has been demonstrated to be associated with liver damage.
However, its potential value in hepatitis B virus (HBV) infection remains unknown.
This study was designed to investigate the role of IL-33 in hydrodynamic HBV mouse model.
Different doses of IL-33 were used to treat HBV wild-type, ST2 knockout, CD8+ T depletion, NK depletion C57BL/6 mice and C.B-17 SCID and nod SCID mouse, respectively.
The concentrations of HBV DNA, HBsAg, HBeAg, and molecules related to liver function were detected in the collected serum at different time points from model mice.
Intrahepatic HBcAg was visualized by immunohistochemical staining of liver tissues.
In vitro, hepG2 cells were transfected with pAAV-HBV 1.2, then treated with IL-33.
The results showed that IL-33 significantly reduced HBV DNA and HBsAg in a dose-dependent manner in HBV wild-type mice.
However, in the IL-33 specific receptor ST2 knockout mice, their antiviral effects could not be exerted.
Through immunodeficient animal models and in vivo immune cell depletion mouse model, we found that IL-33 could not play antiviral effects without NK cells.
Moreover, IL-33 could reduce the levels of HBsAg and HBeAg in the supernatant of HBV-transfected hepG2 cells in vitro.
Our study revealed that IL-33 could inhibit HBV through ST2 receptor in the HBV mouse model, and this effect can be impaired without NK cell.
Additionally, IL-33 had the direct anti-HBV effect in vitro, indicating that IL-33 could be a potent inducer of HBV clearance and a promising drug candidate.
American Psychological Association (APA)
Gao, Xiuzhu& Chi, Xiumei& Wang, Xiaomei& Wu, Ruihong& Xu, Hongqin& Zhan, MengRu…[et al.]. 2020. IL-33 Inhibits Hepatitis B Virus through Its Receptor ST2 in Hydrodynamic HBV Mouse Model. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1191502
Modern Language Association (MLA)
Gao, Xiuzhu…[et al.]. IL-33 Inhibits Hepatitis B Virus through Its Receptor ST2 in Hydrodynamic HBV Mouse Model. Mediators of Inflammation No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1191502
American Medical Association (AMA)
Gao, Xiuzhu& Chi, Xiumei& Wang, Xiaomei& Wu, Ruihong& Xu, Hongqin& Zhan, MengRu…[et al.]. IL-33 Inhibits Hepatitis B Virus through Its Receptor ST2 in Hydrodynamic HBV Mouse Model. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1191502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191502