CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis
Joint Authors
Chi, Xiao-Ling
Chen, Qubo
Lai, Lanmin
Lu, Xinyi
Wu, Huaxian
Sun, Jing
Wu, Weilin
Cai, Li
Zeng, Xuan
Wang, Chuyang
Chen, WeiCheng
Peng, Anping
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
CD19+CD24hiCD38hi B cells are immature transitional B cells that, in normal individuals, exert suppressive effects by IL-10 production but are quantitatively altered and/or functionally impaired in individuals with various autoimmune diseases.
Primary biliary cholangitis (PBC), an autoimmune disease, clinically presents as chronic cholestasis and nonsuppurative destructive cholangitis.
A role for CD19+CD24hiCD38hi B cells in PBC is unknown.
This study investigated the frequency and functional variation of circulating CD19+CD24hiCD38hi B cells in PBC patients.
Flow cytometry was employed to quantify the percentage of CD19+CD24hiCD38hi B cells in peripheral blood samples.
Correlations between CD19+CD24hiCD38hi B cells and routine laboratory parameters were assessed.
Levels of IL-10, TNF-α, IL-6 and IL-12, and Tim-1 in CD19+CD24hiCD38hi B cells from PBC patients were analyzed.
The effect of CD19+CD24hiCD38hi B cells on CD4+T cell differentiation was evaluated.
The percentage of CD19+CD24hiCD38hi B cells in PBC patients was significantly higher than in healthy controls and was positively correlated with liver cholestasis.
After activation by anti-B cell receptor and CpG, the production of IL-10 was decreased and the production of IL-6 and IL-12 was increased in CD19+CD24hiCD38hi B cells from PBC patients.
Moreover, Tim-1 levels were significantly downregulated in CD19+CD24hiCD38hi B cells from PBC patients.
Coculture showed that PBC-derived CD19+CD24hiCD38hi B cells were less capable of CD4+T cell inhibition, but promoted Th1 cell differentiation.
In conclusion, PBC patients have expanded percentages, but impaired CD19+CD24hiCD38hi B cells, which correlate with disease damage.
In PBC patients, this B cell subset has a skewed proinflammatory cytokine profile and a decreased capacity to suppress immune function, which may contribute to the pathogenesis of PBC.
American Psychological Association (APA)
Chen, Qubo& Lai, Lanmin& Chi, Xiao-Ling& Lu, Xinyi& Wu, Huaxian& Sun, Jing…[et al.]. 2020. CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1191621
Modern Language Association (MLA)
Chen, Qubo…[et al.]. CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis. Mediators of Inflammation No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1191621
American Medical Association (AMA)
Chen, Qubo& Lai, Lanmin& Chi, Xiao-Ling& Lu, Xinyi& Wu, Huaxian& Sun, Jing…[et al.]. CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1191621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191621