Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1
Joint Authors
Kubota, Ryo
Reid, Michael J.
Lieu, Kuo Lee
Orme, Mark
Diamond, Christine
Tulberg, Niklas
Henry, Susan H.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Vascular adhesion protein-1 (VAP-1) is an ectoenzyme that functions as a copper-containing amine oxidase and is involved in leukocyte adhesion at sites of inflammation.
Inhibition of VAP-1 oxidative deamination has become an attractive target for anti-inflammatory therapy with demonstrated efficacy in rodent models of inflammation.
A previous comparison of purified recombinant VAP-1 from mouse, rat, monkey, and human gene sequences predicted that rodent VAP-1 would have higher affinity for smaller hydrophilic substrates/inhibitors because of its narrower and more hydrophilic active site channel.
An optimized in vitro oxidative deamination fluorescence assay with benzylamine (BA) was used to compare inhibition of five known inhibitors in recombinant mouse, rat, and human VAP-1.
Human VAP-1 was more sensitive compared to rat or mouse VAP-1 (lowest IC50 concentration) to semicarbazide but was least sensitive to hydralazine and LJP-1207.
Hydralazine had a lower IC50 in rats compared to humans, although not significant.
However, the IC50 of hydralazine was significantly higher in the rat compared to mouse VAP-1.
The larger hydrophobic compounds from Astellas (compound 35c) and Boehringer Ingelheim (PXS-4728A) were hypothesized to have higher binding affinity for human VAP-1 compared to rodent VAP-1 since the channel in human VAP-1 is larger and more hydrophobic than that in rodent VAP-1.
Although the sensitivity of these two inhibitors was the lowest in the mouse enzyme, we found no significant differences between mouse, rat, and human VAP-1.
Michaelis-Menten kinetics of the small primary amines phenylethylamine and tyramine were also compared to the common marker substrate BA demonstrating that BA had the highest affinity among the substrates.
Rat VAP-1 had the highest affinity for all three substrates and mouse VAP-1 had intermediate affinity for BA and phenylethylamine, but tyramine was not a substrate for mouse VAP-1 under these assay conditions.
These results suggest that comparing oxidative deamination in mouse and rat VAP-1 may be important if using these species for preclinical efficacy models.
American Psychological Association (APA)
Kubota, Ryo& Reid, Michael J.& Lieu, Kuo Lee& Orme, Mark& Diamond, Christine& Tulberg, Niklas…[et al.]. 2020. Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1191647
Modern Language Association (MLA)
Kubota, Ryo…[et al.]. Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1. Mediators of Inflammation No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1191647
American Medical Association (AMA)
Kubota, Ryo& Reid, Michael J.& Lieu, Kuo Lee& Orme, Mark& Diamond, Christine& Tulberg, Niklas…[et al.]. Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1191647
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191647