Inhibition of IL-17 and IL-23 in Human Keratinocytes by the A3 Adenosine Receptor Agonist Piclidenoson

Joint Authors

Cohen, Shira
Barer, Faina
Fishman, Pnina
Itzhak, Inbal
Silverman, Michael H.

Source

Journal of Immunology Research

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-05-15

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Biology

Abstract EN

Interleukin-17 and interleukin-23 play major roles in the inflammatory process in psoriasis.

The Gi protein-associated A3 adenosine receptor (A3AR) is known to be overexpressed in inflammatory cells and in peripheral blood mononuclear cells (PBMCs) of patients with autoimmune inflammatory conditions.

Piclidenoson, a selective agonist at the A3AR, induces robust anti-inflammatory effect in psoriasis patients.

In this study, we aimed to explore A3AR expression levels in psoriasis patients and its role in mediating the anti-inflammatory effect of piclidenoson in human keratinocyte cells.

A3AR expression levels were evaluated in skin tissue and PBMCs derived from psoriasis patients and healthy subjects.

Proliferation assay and the expression of signaling proteins were used to evaluate piclidenoson effect on human keratinocytes (HaCat).

High A3AR expression levels were found in a skin biopsy and in PBMCs from psoriasis patients in comparison to healthy subjects.

Piclidenoson inhibited the proliferation of HaCat cells through deregulation of the NF-κB signaling pathway, leading to a decrease in interleukin-17 and interleukin-23 expression levels.

This effect was counteracted by the specific antagonist MRS 1523.

A3AR overexpression in skin and PBMCs of psoriasis patients may be used as a target to inhibit pathological cell proliferation and the production of interleukin-17 and interleukin-23.

American Psychological Association (APA)

Cohen, Shira& Barer, Faina& Itzhak, Inbal& Silverman, Michael H.& Fishman, Pnina. 2018. Inhibition of IL-17 and IL-23 in Human Keratinocytes by the A3 Adenosine Receptor Agonist Piclidenoson. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1191752

Modern Language Association (MLA)

Cohen, Shira…[et al.]. Inhibition of IL-17 and IL-23 in Human Keratinocytes by the A3 Adenosine Receptor Agonist Piclidenoson. Journal of Immunology Research No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1191752

American Medical Association (AMA)

Cohen, Shira& Barer, Faina& Itzhak, Inbal& Silverman, Michael H.& Fishman, Pnina. Inhibition of IL-17 and IL-23 in Human Keratinocytes by the A3 Adenosine Receptor Agonist Piclidenoson. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1191752

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1191752