Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes
Joint Authors
Midtbö, Kristine
Eklund, Daniel
Särndahl, Eva
Persson, Alexander
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Inflammasomes cleave and activate interleukin- (IL-) 1β and IL-18 which have both shared and unique biological functions.
IL-1β is an important mediator of the acute phase response to infections and tissue damage, whereas IL-18 takes part in activation and tailoring of the adaptive immune response.
While IL-1β has served as the prototypic indicator of inflammasome activation, few studies have compared the potential differences in IL-1β and IL-18 production during inflammasome activation.
Since these cytokines partake in different immune pathways, the involvement of inflammasome activity in different conditions needs to be described beyond IL-1β production alone.
To address a potential heterogeneity in inflammasome functionality, ATP, chitosan, or silica oxide (SiO2) were used to induce NLRP3 inflammasome activation in THP-1 cells and the subsequent outcomes were quantified.
Despite using doses of the inflammasome inducers yielding similar release of IL-1β, SiO2-stimulated cells showed a lower concentration of released IL-18 compared to ATP and chitosan.
Hence, the cells stimulated with SiO2 responded with a distinctly different IL-18 : IL-1β ratio.
The difference in the IL-18 : IL-1β ratio for SiO2 was constant over different doses.
While all downstream responses were strictly dependent on a functional NLRP3 inflammasome, the differences did not depend on the level of gene expression, caspase-1 activity, or pyroptosis.
We suggest that the NLRP3 inflammasome response should be considered a dynamic process, which can be described by taking the ratio between IL-1β and IL-18 into account and moving away from an on/off perspective of inflammasome activation.
American Psychological Association (APA)
Midtbö, Kristine& Eklund, Daniel& Särndahl, Eva& Persson, Alexander. 2020. Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191782
Modern Language Association (MLA)
Midtbö, Kristine…[et al.]. Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes. Mediators of Inflammation No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1191782
American Medical Association (AMA)
Midtbö, Kristine& Eklund, Daniel& Särndahl, Eva& Persson, Alexander. Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191782
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191782