In Vitro Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of Heliotropium europaeum
Joint Authors
Anwar, Mohammad
Achakzai, Jahangir Khan
Kakar, Abdul Manan
Kakar, Shahabuddin
Khan, Javed
Khan, Nazima Yousaf
Akhtar, Basira
Khan, Ghulam Mustafa
Panezai, Marina
Imran, Muhammad
Shahwani, Nisar Ahmed
Achakzai, Tehmina
Akbar, Ali
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-01
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In this study, anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies were evaluated.
The oxidative burst assay using the chemiluminescence technique, MTT assay, brine shrimp lethality assay, and FTIR analysis were the methods used for the evaluation of anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies, respectively.
The whole-plant butanol fraction of Heliotropium europaeum (WBFHE) showed anti-inflammatory activity on ROS having IC50 14.7±2.5 while the extract and other fractions of the whole plant of Heliotropium europaeum exhibited no anti-inflammatory activity.
None of the extract and fractions of the whole plant of Heliotropium europaeum exhibited anticancer (MCF-7, 3T3, and HeLa cell lines) activities.
The whole-plant aqueous fraction of Heliotropium europaeum (WAFHE) and whole-plant butanol fraction of Heliotropium europaeum (WBFHE) showed lethality at high concentration while at low concentration, no toxicity was shown.
The whole-plant methanolic extract of Heliotropium europaeum (WMEHE) and whole-plant n-hexane fraction of Heliotropium europaeum (WHFHE) exhibited no toxicity.
FTIR interpretation showed the functional groups for the aromatic compounds, phenols, carboxylic acids, esters, alkanes, alkenes, alcohols, alkyl halides, sulfate esters, phosphines, silanes, nitriles, thiols, amines, phosphoric acids, and nitro compounds.
American Psychological Association (APA)
Achakzai, Jahangir Khan& Anwar, Mohammad& Akhtar, Basira& Kakar, Shahabuddin& Akbar, Ali& Kakar, Abdul Manan…[et al.]. 2020. In Vitro Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of Heliotropium europaeum. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1191789
Modern Language Association (MLA)
Achakzai, Jahangir Khan…[et al.]. In Vitro Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of Heliotropium europaeum. Mediators of Inflammation No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1191789
American Medical Association (AMA)
Achakzai, Jahangir Khan& Anwar, Mohammad& Akhtar, Basira& Kakar, Shahabuddin& Akbar, Ali& Kakar, Abdul Manan…[et al.]. In Vitro Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of Heliotropium europaeum. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1191789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191789