Metabolic Profiling by UPLC–Orbitrap–MSMS of Liver from C57BL6 Mice with DSS-Induced Inflammatory Bowel Disease
Joint Authors
Xin, Zhongquan
Zhai, Zhenya
Long, Hongrong
Ni, Xiaojun
Deng, Jinping
Yi, Lunzhao
Deng, Baichuan
Zhang, Fan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Liver disorder often occurs in patients with inflammatory bowel disease (IBD); however, the changes in IBD-induced liver disorder at the intrinsic molecular level (chiefly metabolites) and therapeutic targets are still poorly characterized.
First, a refined and translationally relevant model of DSS chronic colitis in C57BL/6 mice was established, and cecropin A and antibiotics were used as interventions.
We found that the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in the liver tissues of mice were highly increased in the context of DSS treatment but were lowered by cecropin A and antibiotics.
Subsequently, an untargeted metabolomics analysis was performed by UPLC–Orbitrap–MS/MS to reveal the metabolic profile and attempt to find the potential therapeutic targets of the liver disorders that occur in IBD.
Notably, 133 metabolites were identified by an integrated database.
Metabolism network and pathway analyses demonstrated that the metabolic disturbance of the liver in IBD mice was mainly enriched in bile acid metabolism, arachidonic acid metabolism, amino acid metabolism, and steroid hormone biosynthesis, while those disturbances were regulated or reversed through cecropin A and antibiotic treatment.
Furthermore, the top 20 metabolites, such as glutathione, maltose, arachidonic acid, and thiamine, were screened as biomarkers via one-way analysis of variance (one-way ANOVA, p<0.05) coupled with variable importance for project values (VIP >1) of orthogonal partial least-squares discriminant analysis (OPLS-DA), which could be upregulated or downregulated with the cecropin A and antibiotics treatment.
Spearman correlation analysis showed that the majority of the biomarkers have a significant correlation with cytokines (TNF-α, IL-1β, IL-6, and IL-10), indicating that those biomarkers may act as potential targets to interact directly or indirectly with cecropin A and antibiotics to affect liver inflammation.
Collectively, our results extend the understanding of the molecular alteration of liver disorders occurring in IBD and offer an opportunity for discovering potential therapeutic targets in the IBD process.
American Psychological Association (APA)
Xin, Zhongquan& Zhai, Zhenya& Long, Hongrong& Zhang, Fan& Ni, Xiaojun& Deng, Jinping…[et al.]. 2020. Metabolic Profiling by UPLC–Orbitrap–MSMS of Liver from C57BL6 Mice with DSS-Induced Inflammatory Bowel Disease. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1191857
Modern Language Association (MLA)
Xin, Zhongquan…[et al.]. Metabolic Profiling by UPLC–Orbitrap–MSMS of Liver from C57BL6 Mice with DSS-Induced Inflammatory Bowel Disease. Mediators of Inflammation No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1191857
American Medical Association (AMA)
Xin, Zhongquan& Zhai, Zhenya& Long, Hongrong& Zhang, Fan& Ni, Xiaojun& Deng, Jinping…[et al.]. Metabolic Profiling by UPLC–Orbitrap–MSMS of Liver from C57BL6 Mice with DSS-Induced Inflammatory Bowel Disease. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1191857
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191857