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Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis
Joint Authors
Braumüller, Sonja
Gebhard, F.
Kalbitz, Miriam
Lackner, Ina
Weber, Birte
Chakraborty, Shinjini
Huber-Lang, Markus S.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Sepsis is associated with global cardiac dysfunction and with high mortality rate.
The development of septic cardiomyopathy is due to complex interactions of damage-associated molecular patters, cytokines, and complement activation products.
The aim of this study was to define the effects of sepsis on cardiac structure, gap junction, and tight junction (TJ) proteins.
Sepsis was induced by cecal ligation and puncture in male C57BL/6 mice.
After a period of 24 h, the expression of cardiac structure, gap junction, and TJ proteins was determined.
Murine HL-1 cells were stimulated with LPS, and mRNA expression of cardiac structure and gap junction proteins, intracellular reactive oxygen species, and troponin I release was analyzed.
Furthermore, pyrogenic receptor subtype 7 (P2X7) expression and troponin I release of human cardiomyocytes (iPS) were determined after LPS exposure.
In vivo, protein expression of connexin43 and α-actinin was decreased after the onset of polymicrobial sepsis, whereas in HL-1 cells, mRNA expression of connexin43, α-actinin, and desmin was increased in the presence of LPS.
Expression of TJ proteins was not affected in vivo during sepsis.
Although the presence of LPS and nigericin resulted in a significant troponin I release from HL-1 cells.
Sepsis affected cardiac structure and gap junction proteins in mice, potentially contributing to compromised cardiac function.
American Psychological Association (APA)
Lackner, Ina& Weber, Birte& Chakraborty, Shinjini& Braumüller, Sonja& Huber-Lang, Markus S.& Gebhard, F.…[et al.]. 2020. Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1191860
Modern Language Association (MLA)
Lackner, Ina…[et al.]. Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis. Mediators of Inflammation No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1191860
American Medical Association (AMA)
Lackner, Ina& Weber, Birte& Chakraborty, Shinjini& Braumüller, Sonja& Huber-Lang, Markus S.& Gebhard, F.…[et al.]. Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1191860
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191860