![](/images/graphics-bg.png)
CypB-CD147 Signaling Is Involved in Crosstalk between Cartilage and FLS in Collagen-Induced Arthritis
Joint Authors
Fan, Kai-Jian
Wang, Qi-Shan
Xu, Bing-Xin
Wang, Ting-Yu
Wu, Jing
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
To investigate the crosstalk between cartilage and fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA), we adopted an in vitro coculture system model of collagen-induced arthritis (CIA) cartilage and CIA FLS monolayer.
CIA rat samples of the synovium and femur head were collected for isolation of FLS and coculture system.
Cartilages were treated with vehicle (Ctrl group), 10 ng/mL interleukin- (IL-) 1α (IL-1α group), and 10 ng/mL IL-1α plus 10 μM dexamethasone (Dex group) for 3 days before coculture with FLS for further 2 days.
After the coculture, FLS were collected to determine the influences of articular cartilage on synoviocytes.
Whether the CypB-CD147 signaling pathway is involved in the interactions between cartilage and FLS is assayed.
Results showed that IL-1α-stimulated CIA cartilage promoted the proliferation and reduced the apoptosis of FLS.
Increased inflammatory cytokines and decreased p57 expression were found in cocultured FLS stimulated by IL-1α-challenged CIA cartilage.
Upregulation of NF-κB and I-κB kinase β (IKK-β) and downregulation of the inhibitor of NF-κBα (I-κBα) protein were observed in cocultured FLS.
After coculture, significant increases in the expression of cyclophilin B (CypB) and CD147 were observed in CIA cartilage and FLS, respectively.
Furthermore, results of immunofluorescence staining showed that the anti-CD147 antibody significantly suppressed p65 nuclear translocation in cocultured FLS stimulated by IL-1α-challenged CIA cartilage.
In conclusion, inflammatory effects in the cartilage-FLS coculture system are associated with the CypB-CD147 mediating NF-κB pathway which may further enhance the inflammation in RA.
American Psychological Association (APA)
Wang, Qi-Shan& Xu, Bing-Xin& Fan, Kai-Jian& Wu, Jing& Wang, Ting-Yu. 2020. CypB-CD147 Signaling Is Involved in Crosstalk between Cartilage and FLS in Collagen-Induced Arthritis. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1191909
Modern Language Association (MLA)
Wang, Qi-Shan…[et al.]. CypB-CD147 Signaling Is Involved in Crosstalk between Cartilage and FLS in Collagen-Induced Arthritis. Mediators of Inflammation No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1191909
American Medical Association (AMA)
Wang, Qi-Shan& Xu, Bing-Xin& Fan, Kai-Jian& Wu, Jing& Wang, Ting-Yu. CypB-CD147 Signaling Is Involved in Crosstalk between Cartilage and FLS in Collagen-Induced Arthritis. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1191909
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191909