Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity
Joint Authors
Chen, Miaomiao
Zuo, Xiaoshuang
Xu, Qinqin
Wang, Rong
Fan, Suhua
Wu, Hai
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nanodiamonds (NDs) have been recognized as emerging carbon-based delivery vehicles due to their biocompatibility.
NDs were reported to be nontoxic and suited for biomedical applications in the complete cell culture medium.
However, in this study, the cytotoxicity of NDs in serum-free medium was studied and indicated that serum proteins in cell culture medium played significant effect on the toxicity of NDs.
Therefore, the interaction mechanism between NDs and a serum protein (human serum albumin, HSA) was first investigated by fluorescence quenching technique and circular dichroism (CD) spectrometry.
The results suggest that HSA strongly bonds on the NDs surface to form “protein corona,” which not only prevents the aggregation of NDs and improves its stability but also inhibits the cytotoxicity of NDs.
In serum-free medium, NDs exhibited obvious toxicity toward the human lung epithelial cell line (BEAS-2B) and showed concentration-dependent cytotoxicity.
In the presence of bovine serum albumin (BSA), which shares structural homology and similar properties with HSA, toxicity of NDs was apparently inhibited.
Therefore, the interaction between serum protein and NDs should be considered in the understanding of the biological effects of NDs exposure in biomedical applications.
American Psychological Association (APA)
Chen, Miaomiao& Zuo, Xiaoshuang& Xu, Qinqin& Wang, Rong& Fan, Suhua& Wu, Hai. 2019. Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity. Journal of Spectroscopy،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1192039
Modern Language Association (MLA)
Chen, Miaomiao…[et al.]. Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity. Journal of Spectroscopy No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1192039
American Medical Association (AMA)
Chen, Miaomiao& Zuo, Xiaoshuang& Xu, Qinqin& Wang, Rong& Fan, Suhua& Wu, Hai. Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity. Journal of Spectroscopy. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1192039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192039