Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810
Joint Authors
Gulbe, G.
Pilmane, Māra
Saulīte, Vaira
Doniņa, Simona
Jermolajevs, Jevgenijs
Peškova, Lilija
Valdovska, Anda
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis.
The composition GLP 810 consists of lactic acid, lysozyme, glycopeptides, and 0.9% solution of NaCl.
The following parameters were studied: (1) leukocyte differential distribution in milk, (2) expression of cytokines in milk leukocytes, (3) antibacterial activity, and (4) milk quality.
Nineteen mammary glands in five lactating cows were infused with 10 mL of GLP 810, and nineteen other glands from five control cows were treated with 10 mL 0.9% NaCl.
The results showed that after intramammary administration of the composition GLP 810 three times with 48 h intervals, the following effects on leukocyte populations in milk were observed: (1) an increase in the number of polymorphonuclear leukocytes and lymphocytes and (2) a decrease in the number of macrophages.
A reduction in the number of pathogenic bacteria was also detected.
The analyses of tumour necrosis factor-alpha, interleukin-10, and beta-defensin-2 revealed that the production of the aforementioned cytokines significantly increased, whereas no significant effects on interleukin-1 and caspase-6 expression in milk leukocytes were recorded.
However, there were significant differences between mammary glands with high and low milk somatic cell count.
The results suggest that the composition GLP 810 has an immunomodulatory effect on mammary glands and it could be used for improving the immune response in cows with subclinical mastitis during lactation.
American Psychological Association (APA)
Gulbe, G.& Pilmane, Māra& Saulīte, Vaira& Doniņa, Simona& Jermolajevs, Jevgenijs& Peškova, Lilija…[et al.]. 2020. Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1192050
Modern Language Association (MLA)
Gulbe, G.…[et al.]. Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810. Mediators of Inflammation No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1192050
American Medical Association (AMA)
Gulbe, G.& Pilmane, Māra& Saulīte, Vaira& Doniņa, Simona& Jermolajevs, Jevgenijs& Peškova, Lilija…[et al.]. Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1192050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192050