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Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
Joint Authors
Helmin-Basa, Anna
Gackowska, Lidia
Socha, Piotr
Szalecki, Mieczysław
Trojanek, Joanna B.
Michałkiewicz, Jacek
Grzywa-Czuba, Renata
Jańczyk, Wojciech
Wierzbicka-Rucińska, Aldona
Kubiszewska, Izabela
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-10
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Gene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD).
Gene expression patterns were correlated with their plasma protein counterparts, systemic parameters of liver injury, and selected markers of inflammation.
The MMP-2, MMP-9, MMP-12, MMP-14, TIMP-1, TIMP-2, TGF-β, and IL-6 transcripts levels were tested by the real-time PCR.
Plasma concentrations of MMP-9, TIMP-1, MMP-9/TIMP-1 ratio, MMP-2/TIMP-2 ratio, sCD14, leptin, resistin, IL-1 beta, and IL-6 and serum markers of liver injury were estimated by ELISA.
The MMP-9, TIMP-2 expression levels, plasma amounts of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio were increased in children with NAFLD.
Concentrations of AST, ALT, GGT, and leptin were elevated in serum patients with NAFLD, while concentration of other inflammatory or liver injury markers was unchanged.
The MMP-2 and MMP-9 levels correlated with serum liver injury parameters (ALT and GGT concentrations, respectively); there were no other correlations between MMP/TIMP gene expression profiles, their plasma counterparts, and serum inflammatory markers.
Association of MMP-2 and MMP-9 expression with serum liver injury parameters (ALT, GGT) may suggest leukocyte engagement in the early stages of NAFLD development which possibly precedes subsequent systemic inflammatory responses.
American Psychological Association (APA)
Trojanek, Joanna B.& Michałkiewicz, Jacek& Grzywa-Czuba, Renata& Jańczyk, Wojciech& Gackowska, Lidia& Kubiszewska, Izabela…[et al.]. 2020. Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1192068
Modern Language Association (MLA)
Trojanek, Joanna B.…[et al.]. Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease. Mediators of Inflammation No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1192068
American Medical Association (AMA)
Trojanek, Joanna B.& Michałkiewicz, Jacek& Grzywa-Czuba, Renata& Jańczyk, Wojciech& Gackowska, Lidia& Kubiszewska, Izabela…[et al.]. Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1192068
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192068