Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves
Joint Authors
Huang, Chun-Ming
Liu, Pei-Feng
Wang, Yanhan
Ulrich, Robert G.
Simmons, Christopher W.
VanderGheynst, Jean S.
Gallo, Richard L.
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Transgene introgression is a major concern associated with transgenic plant-based vaccines.
Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression.
Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativus L.), resulting in transient expression and accumulation of SEB in planta.
This approach can simultaneously express multiple antigens in a single leaf.
Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrating multiple radish leaves using a multichannel pipette.
The expression of SEB was detectable in two leaf cell types (epidermal and guard cells) in agroinfiltrated leaves.
ICR mice intranasally immunized with homogenized leaves agroinfiltrated with SEB elicited detectable antibody to SEB and displayed protection against SEB-induced interferon-gamma (IFN-γ) production.
The concept of encapsulating antigens in leaves rather than purifying them for immunization may facilitate rapid vaccine production during an epidemic disease.
American Psychological Association (APA)
Liu, Pei-Feng& Wang, Yanhan& Ulrich, Robert G.& Simmons, Christopher W.& VanderGheynst, Jean S.& Gallo, Richard L.…[et al.]. 2018. Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1192157
Modern Language Association (MLA)
Liu, Pei-Feng…[et al.]. Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves. Journal of Immunology Research No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1192157
American Medical Association (AMA)
Liu, Pei-Feng& Wang, Yanhan& Ulrich, Robert G.& Simmons, Christopher W.& VanderGheynst, Jean S.& Gallo, Richard L.…[et al.]. Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1192157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192157