Leptin Promoted IL-17 Production from ILC2s in Allergic Rhinitis
Joint Authors
Zeng, Qingxiang
Luo, Xi
Tang, Yiquan
Liu, Wenlong
Wen, Yanhui
Ma, Renqiang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Interleukin-17 plays important roles in allergic diseases.
Several studies proved that leptin promoted Th17 immune responses by inducing RORγt transcription.
ILC2 is an important member of the early stage of immune response.
Therefore, we aimed to explore the effect of leptin on the IL-17 production by ILC2 in AR in this study.
Methods.
Fifteen AR patients and fifteen healthy controls were enrolled.
Serum leptin levels were measured, and their correlation with the frequency of IL-17+ ILC2 cells was analyzed using enzyme-linked immunosorbent assay (ELISA) and flow cytometry.
ILC2 was stimulated by leptin, and the expression of IL-17, IL-5, and IL-13 was detected by ELISA.
The correlated pathways were confirmed by real-time PCR.
Results.
We found that serum leptin and the frequency of IL-17-producing ILC2s in AR were significantly higher compared with those in controls.
After being incubated with leptin, the frequency of IL-17+ ILC2 cells and IL-17 production from ILC2 was upregulated compared with that in controls.
We also found that leptin induced RORγt and Ahr expression by ILC2.
Moreover, leptin-induced IL-17-producing ILC2 concomitantly expressed IL-5 and IL-13.
Conclusions.
Our data provide preliminary evidence that leptin-induced IL-17 production from ILC2 cells is dependent on RORγt and Ahr expression and the blockade of leptin may be a promising target for the treatment of AR.
American Psychological Association (APA)
Wen, Yanhui& Zeng, Qingxiang& Luo, Xi& Ma, Renqiang& Tang, Yiquan& Liu, Wenlong. 2020. Leptin Promoted IL-17 Production from ILC2s in Allergic Rhinitis. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1192206
Modern Language Association (MLA)
Wen, Yanhui…[et al.]. Leptin Promoted IL-17 Production from ILC2s in Allergic Rhinitis. Mediators of Inflammation No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1192206
American Medical Association (AMA)
Wen, Yanhui& Zeng, Qingxiang& Luo, Xi& Ma, Renqiang& Tang, Yiquan& Liu, Wenlong. Leptin Promoted IL-17 Production from ILC2s in Allergic Rhinitis. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1192206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192206