Combination Therapy with EpCAM-CAR-NK-92 Cells and Regorafenib against Human Colorectal Cancer Models
Joint Authors
Zhang, Qing
Zhang, Haixu
Ding, Jiage
Liu, Hongyan
Li, Huizhong
Li, Hailong
Lu, Mengmeng
Miao, Yangna
Li, Liantao
Zheng, Junnian
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Adoptive chimeric antigen receptor-modified T or NK cells (CAR-T or CAR-NK) offer new options for cancer treatment.
CAR-T therapy has achieved encouraging breakthroughs in the treatment of hematological malignancies.
However, their therapeutic efficacy against solid tumors is limited.
New regimens, including combinations with chemical drugs, need to be studied to enhance the therapeutic efficacy of CAR-T or NK cells for solid tumors.
An epithelial cell adhesion molecule- (EpCAM-) specific second-generation CAR was constructed and transduced into NK-92 cells by lentiviral vectors.
Immune effects, including cytokine release and cytotoxicity of the CAR-NK-92 cells against EpCAM-positive colon cancer cells, were evaluated in vitro.
Synergistic effects of regorafenib and CAR-NK-92 cells were analyzed in a mouse model with human colorectal cancer xenografts.
The CAR-NK-92 cells can specifically recognize EpCAM-positive colorectal cancer cells and release cytokines, including IFN-γ, perforin, and granzyme B, and show specific cytotoxicity in vitro.
The growth suppression efficacy of combination therapy with regorafenib and CAR-NK-92 cells on established EpCAM-positive tumor xenografts was more significant than that of monotherapy with CAR-NK-92 cells or regorafenib.
Our results provided a novel strategy to treat colorectal cancer and enhance the therapeutic efficacy of CAR-modified immune effector cells for solid tumors.
American Psychological Association (APA)
Zhang, Qing& Zhang, Haixu& Ding, Jiage& Liu, Hongyan& Li, Huizhong& Li, Hailong…[et al.]. 2018. Combination Therapy with EpCAM-CAR-NK-92 Cells and Regorafenib against Human Colorectal Cancer Models. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1192296
Modern Language Association (MLA)
Zhang, Qing…[et al.]. Combination Therapy with EpCAM-CAR-NK-92 Cells and Regorafenib against Human Colorectal Cancer Models. Journal of Immunology Research No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1192296
American Medical Association (AMA)
Zhang, Qing& Zhang, Haixu& Ding, Jiage& Liu, Hongyan& Li, Huizhong& Li, Hailong…[et al.]. Combination Therapy with EpCAM-CAR-NK-92 Cells and Regorafenib against Human Colorectal Cancer Models. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1192296
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192296