Decrease in Intracellular Perforin Levels and IFN-γ Production in Human CD8+ T Cell Line following Long-Term Exposure to Asbestos Fibers
Joint Authors
Lee, Suni
Otsuki, Takemi
Yoshitome, Kei
Matsuzaki, Hidenori
Nishimura, Yasumitsu
Kumagai-Takei, Naoko
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Although the tumorigenicity of asbestos, which is thought to cause mesothelioma, has been clarified, its effect on antitumor immunity requires further investigation.
We previously reported a decrease in the percentage of perforin+ cells of stimulated CD8+ lymphocytes derived from patients with malignant mesothelioma.
Therefore, we examined the effects of long-term exposure to asbestos on CD8+ T cell functions by comparing long-term cultures of the human CD8+ T cell line EBT-8 with and without exposure to chrysotile (CH) asbestos as an in vitro model.
Exposure to CH asbestos at 5 μg/ml or 30 μg/ml did not result in a decrease in intracellular granzyme B in EBT-8 cells.
In contrast, the percentage of perforin+ cells decreased at both doses of CH exposure.
CH exposure at 30 μg/ml did not suppress degranulation following stimulation with antibodies to CD3.
Secreted production of IFN-γ stimulated via CD3 decreased by CH exposure at 30 μg/ml, although the percentage of IFN-γ+ cells induced by PMA/ionomycin did not decrease.
These results indicate that long-term exposure to asbestos can potentially suppress perforin levels and the production of IFN-γ in human CD8+ T cells.
American Psychological Association (APA)
Kumagai-Takei, Naoko& Nishimura, Yasumitsu& Matsuzaki, Hidenori& Lee, Suni& Yoshitome, Kei& Otsuki, Takemi. 2018. Decrease in Intracellular Perforin Levels and IFN-γ Production in Human CD8+ T Cell Line following Long-Term Exposure to Asbestos Fibers. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192323
Modern Language Association (MLA)
Kumagai-Takei, Naoko…[et al.]. Decrease in Intracellular Perforin Levels and IFN-γ Production in Human CD8+ T Cell Line following Long-Term Exposure to Asbestos Fibers. Journal of Immunology Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1192323
American Medical Association (AMA)
Kumagai-Takei, Naoko& Nishimura, Yasumitsu& Matsuzaki, Hidenori& Lee, Suni& Yoshitome, Kei& Otsuki, Takemi. Decrease in Intracellular Perforin Levels and IFN-γ Production in Human CD8+ T Cell Line following Long-Term Exposure to Asbestos Fibers. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192323
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192323