Melittin Constrains the Expression of Identified Key Genes Associated with Bladder Cancer
Joint Authors
Li, Bai
Xie, Nianlin
Cai, Li-Bo
Jin, Zidan
Yao, Jie
Qi, Shuai
Zhang, Zhan
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-03
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
This work is aimed at investigating the effect of melittin on identified key genes in bladder cancer (BC) and further providing a theoretical basis for BC treatment.
GSE35014 downloaded from the Gene Expression Omnibus (GEO) database was used to screen differentially expressed genes (DEGs) in BC cells and control.
Results showed that a total of 389 upregulated and 169 downregulated genes were identified.
Subsequently, GO analysis, KEGG pathway enrichment analysis, and PPI network analysis were employed to disclose the crucial genes and signaling pathways involved in BC.
Fifteen module-related DEGs and their associated signaling pathways were obtained according to the PPI network and modular analyses.
Based on the analysis of articles retrieved in the PubMed database, we found that melittin could induce apoptosis and constrain the progression of tumor cells as a result of regulating critical cancer-related signaling pathways, such as PI3K-Akt and TNF signaling pathways.
Furthermore, PI3K-Akt and TNF signaling pathways were also found to be associated with module-related DEGs according to biological analyses.
At last, qRT-PCR analysis demonstrated that melittin could constrain the expression of module-related DEGs (LPAR1, COL5A1, COL6A2, CXCL1, CXCL2, and CXCL3) associated with PI3K-Akt and TNF signaling pathways in BC cells.
Functional assays revealed that melittin could constrain the proliferative and migrated abilities of BC cells.
Conjointly, these findings provide a theoretical basis for these six genes as drug-sensitive markers of melittin in BC treatment.
American Psychological Association (APA)
Jin, Zidan& Yao, Jie& Xie, Nianlin& Cai, Li-Bo& Qi, Shuai& Zhang, Zhan…[et al.]. 2018. Melittin Constrains the Expression of Identified Key Genes Associated with Bladder Cancer. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1192447
Modern Language Association (MLA)
Jin, Zidan…[et al.]. Melittin Constrains the Expression of Identified Key Genes Associated with Bladder Cancer. Journal of Immunology Research No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1192447
American Medical Association (AMA)
Jin, Zidan& Yao, Jie& Xie, Nianlin& Cai, Li-Bo& Qi, Shuai& Zhang, Zhan…[et al.]. Melittin Constrains the Expression of Identified Key Genes Associated with Bladder Cancer. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1192447
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192447