Inhibition of MAPK and STAT3-SOCS3 by Sakuranetin Attenuated Chronic Allergic Airway Inflammation in Mice
Joint Authors
Prado, Carla M.
Caperuto, Luciana Chagas
Santana, Fernanda Paula R.
Arantes-Costa, Fernanda Magalhães
Ghilardi Lago, João Henrique
Rodrigues Pinheiro, Aruanã Joaquim Matheus
da Silva, Rafael C.
Grecco, Simone dos S.
Claudio, Samuel R.
Yoshizaki, Kelly
Macchione, Mariângela
Ribeiro, Daniel A.
Lima-Neto, Lídio G.
Tibério, Iolanda F. L. C.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-03
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Asthma allergic disease is caused by airway chronic inflammation.
Some intracellular signaling pathways, such as MAPK and STAT3-SOCS3, are involved in the control of airway inflammation in asthma.
The flavonoid sakuranetin demonstrated an anti-inflammatory effect in different asthma models.
Our aim was to clarify how sakuranetin treatment affects MAPK and STAT3-SOCS3 pathways in a murine experimental asthma model.
Mice were submitted to an asthma ovalbumin-induction protocol and were treated with vehicle, sakuranetin, or dexamethasone.
We assayed the inflammatory profile, mucus production, and serum antibody, STAT3-SOCS3, and MAPK levels in the lungs.
Morphological alterations were also evaluated in the liver.
LPS-stimulated RAW 264.7 cells were used to evaluate the effects of sakuranetin on nitric oxide (NO) and cytokine production.
In vivo, sakuranetin treatment reduced serum IgE levels, lung inflammation (eosinophils, neutrophils, and Th2/Th17 cytokines), and respiratory epithelial mucus production in ovalbumin-sensitized animals.
Considering possible mechanisms, sakuranetin inhibits the activation of ERK1/2, JNK, p38, and STAT3 in the lungs.
No alterations were found in the liver for treated animals.
Sakuranetin did not modify in vitro cell viability in RAW 264.7 and reduced NO release and gene expression of IL-1β and IL-6 induced by LPS in these cells.
In conclusion, our data showed that the inhibitory effects of sakuranetin on eosinophilic lung inflammation can be due to the inhibition of Th2 and Th17 cytokines and the inhibition of MAPK and STAT3 pathways, reinforcing the idea that sakuranetin can be considered a relevant candidate for the treatment of inflammatory allergic airway disease.
American Psychological Association (APA)
Santana, Fernanda Paula R.& da Silva, Rafael C.& Grecco, Simone dos S.& Rodrigues Pinheiro, Aruanã Joaquim Matheus& Caperuto, Luciana Chagas& Arantes-Costa, Fernanda Magalhães…[et al.]. 2019. Inhibition of MAPK and STAT3-SOCS3 by Sakuranetin Attenuated Chronic Allergic Airway Inflammation in Mice. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1192591
Modern Language Association (MLA)
Santana, Fernanda Paula R.…[et al.]. Inhibition of MAPK and STAT3-SOCS3 by Sakuranetin Attenuated Chronic Allergic Airway Inflammation in Mice. Mediators of Inflammation No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1192591
American Medical Association (AMA)
Santana, Fernanda Paula R.& da Silva, Rafael C.& Grecco, Simone dos S.& Rodrigues Pinheiro, Aruanã Joaquim Matheus& Caperuto, Luciana Chagas& Arantes-Costa, Fernanda Magalhães…[et al.]. Inhibition of MAPK and STAT3-SOCS3 by Sakuranetin Attenuated Chronic Allergic Airway Inflammation in Mice. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1192591
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192591