Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro
Joint Authors
Zonfrillo, Manuela
Fuggetta, Maria Pia
Villivà, Cristina
Ravagnan, Giampiero
Bonmassar, Enzo
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
Obesity is considered a clinic condition characterized by a state of chronic low-grade inflammation.
The role of macrophages and adipocytokines in adipose tissue inflammation is in growing investigation.
The physiopathological mechanisms involved in inflammatory state in obesity are not fully understood though the adipocytokines seem to characterize the biochemical link between obesity and inflammation.
The aim of this work is to analyze the effect of theobromine, a methylxanthine present in the cocoa, on adipogenesis and on proinflammatory cytokines evaluated in a model of fat tissue inflammation in vitro.
Methods.
In order to mimic in vitro this inflammatory condition, we investigated the interactions between human-like macrophages U937 and human adipocyte cell lines SGBS.
The effect of theobromine on in vitro cell growth, cell cycle, adipogenesis, and cytokines release in the supernatants has been evaluated.
Results.
Theobromine significantly inhibits the differentiation of preadipocytes in mature adipocytes and reduces the levels of proinflammatory cytokines as MCP-1 and IL-1β in the supernatants obtained by the mature adipocytes and macrophages interaction.
Conclusion.
Theobromine reduces adipogenesis and proinflammatory cytokines; these data suggest its potential therapeutic effect for treating obesity by control of macrophages infiltration in adipose tissue and inflammation.
American Psychological Association (APA)
Fuggetta, Maria Pia& Zonfrillo, Manuela& Villivà, Cristina& Bonmassar, Enzo& Ravagnan, Giampiero. 2019. Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1192601
Modern Language Association (MLA)
Fuggetta, Maria Pia…[et al.]. Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro. Mediators of Inflammation No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1192601
American Medical Association (AMA)
Fuggetta, Maria Pia& Zonfrillo, Manuela& Villivà, Cristina& Bonmassar, Enzo& Ravagnan, Giampiero. Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1192601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192601