High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells
Joint Authors
Xiong, Ying
LaRue, Amanda C.
Sidles, Sara J.
Young, M. Rita I.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-28
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Chronic inflammation is evident in the adipose tissue and periphery of patients with obesity, as well as mouse models of obesity.
T cell subsets in obese adipose tissue are skewed towards Th1- and Th17-associated phenotypes and their secreted cytokines contribute to obesity-associated inflammation.
Our lab recently identified a novel, myeloid-derived CD45+DDR2+ cell subset that modulates T cell activity.
The current study sought to determine how these myeloid-derived CD45+DDR2+ cells are altered in the adipose tissue and peripheral blood of preobese mice and how this population modulates T cell activity.
C57BL/6 mice were fed with a diet high in milkfat (60%·kcal, HFD) ad libitum until a 20% increase in total body weight was reached, and myeloid-derived CD45+DDR2+ cells and CD4+ T cells in visceral adipose tissue (VAT), mammary gland-associated adipose tissue (MGAT), and peripheral blood (PB) were phenotypically analyzed.
Also analyzed was whether mediators from MGAT-primed myeloid-derived CD45+DDR2+ cells stimulate normal CD4+ T cell cytokine production.
A higher percentage of myeloid-derived CD45+DDR2+ cells expressed the activation markers MHC II and CD80 in both VAT and MGAT of preobese mice.
CD4+ T cells were preferentially skewed towards Th1- and Th17-associated phenotypes in the adipose tissue and periphery of preobese mice.
In vitro, MGAT from HFD-fed mice triggered myeloid-derived CD45+DDR2+ cells to induce CD4+ T cell IFN-γ and TNF-α production.
Taken together, this study shows that myeloid-derived CD45+DDR2+ cells express markers of immune activation and suggests that they play an immune modulatory role in the adipose tissue of preobese mice.
American Psychological Association (APA)
Sidles, Sara J.& Xiong, Ying& Young, M. Rita I.& LaRue, Amanda C.. 2019. High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1192625
Modern Language Association (MLA)
Sidles, Sara J.…[et al.]. High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells. Mediators of Inflammation No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1192625
American Medical Association (AMA)
Sidles, Sara J.& Xiong, Ying& Young, M. Rita I.& LaRue, Amanda C.. High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1192625
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192625