Immature Dendritic Cell Therapy Confers Durable Immune Modulation in an Antigen-Dependent and Antigen-Independent Manner in Nonobese Diabetic Mice
Joint Authors
Xia, Chang-Qing
Lo, Jeannette
Peng, Ruihua
Clare-Salzler, Michael J.
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Dendritic cell (DC) immunotherapy has been effective for prevention of type 1 diabetes (T1D) in NOD mice but fails to protect if initiated after active autoimmunity.
As autoreactivity expands inter- and intramolecularly during disease progression, we investigated whether DCs unpulsed or pulsed with β cell antigenic dominant determinants (DD), subdominant determinants (SD), and ignored determinants (ID) could prevent T1D in mice with advanced insulitis.
We found that diabetes was significantly delayed by DC therapy.
Of interest, DCs pulsed with SD or ID appeared to provide better protection.
T lymphocytes from DC-treated mice acquired spontaneous proliferating capability during in vitro culture, which could be largely eliminated by IL-2 neutralizing antibodies.
This trend maintained even 29 weeks after discontinuing DC therapy and appeared antigen-independent.
Furthermore, CD4+Foxp3+ T regulatory cells (Tregs) from DC-treated mice proliferated more actively in vitro compared to the controls, and Tregs from DC-treated mice showed significantly enhanced immunosuppressive activities in contrast to those from the controls.
Our study demonstrates that DC therapy leads to long-lasting immunomodulatory effects in an antigen-dependent and antigen-independent manner and provides evidence for peptide-based intervention during a clinically relevant window to guide DC-based immunotherapy for autoimmune diabetes.
American Psychological Association (APA)
Lo, Jeannette& Xia, Chang-Qing& Peng, Ruihua& Clare-Salzler, Michael J.. 2018. Immature Dendritic Cell Therapy Confers Durable Immune Modulation in an Antigen-Dependent and Antigen-Independent Manner in Nonobese Diabetic Mice. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1192627
Modern Language Association (MLA)
Lo, Jeannette…[et al.]. Immature Dendritic Cell Therapy Confers Durable Immune Modulation in an Antigen-Dependent and Antigen-Independent Manner in Nonobese Diabetic Mice. Journal of Immunology Research No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1192627
American Medical Association (AMA)
Lo, Jeannette& Xia, Chang-Qing& Peng, Ruihua& Clare-Salzler, Michael J.. Immature Dendritic Cell Therapy Confers Durable Immune Modulation in an Antigen-Dependent and Antigen-Independent Manner in Nonobese Diabetic Mice. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1192627
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192627