A Novel Bimodal Imaging Agent Targeting HER2 Molecule of Breast Cancer
Joint Authors
Zhang, Jian
Lv, Wei
Shen, Yamei
Yang, Hengli
Yang, Rui
Cai, Wenbin
Yuan, Lijun
Duan, Yunyou
Zhang, Li
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Nanobubble (NB), a newly developed nanoscaled ultrasound contrast agent (UCA) for molecular imaging, has been widely researched for these years.
Targeting it with functional molecule, nanobubble can adhere selectively to cellular epitopes and receptors outside the vasculature via enhanced permeability and retention (EPR) effect of tumor blood vessel.
To enhance the targeting rate of our previous prepared NBs-Affibody for HER2 (+) breast cancer imaging, we introduced a near-infrared fluorescent (NIRF) dye, IR783, in this study to enhance tumor-specific targeting rate and provide a promising modality for dual-mode imaging.
The prepared IR783-NBs-Affibody presented a uniform nanoscale size around 482.7 ± 54.3 nm, good biosecurity, and stability over time.
The encapsulation efficiency (EE) of IR-783 was 15.09% in the conjugates leading to a successful NIR fluorescence and ultrasound enhancement imaging ex vivo.
IR783-NBs-Affibody was able to automatically accumulate on BT474 cells with a highly increased targeting rate of 85.4% compared with previous NBs-Affibody of 26.6%, while Affibody-guided HER2 binding was only found in HER2-positive cell lines (BT474 and T-47D).
The newly developed IR783-NBs-Affibody is characterized with favorable HER2 targeting ability and bimodal imaging capability for breast cancer.
Thus, IR783-NBs-Affibody holds great potential in molecular diagnosis for patients with breast cancer.
American Psychological Association (APA)
Lv, Wei& Shen, Yamei& Yang, Hengli& Yang, Rui& Cai, Wenbin& Zhang, Jian…[et al.]. 2018. A Novel Bimodal Imaging Agent Targeting HER2 Molecule of Breast Cancer. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192735
Modern Language Association (MLA)
Lv, Wei…[et al.]. A Novel Bimodal Imaging Agent Targeting HER2 Molecule of Breast Cancer. Journal of Immunology Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1192735
American Medical Association (AMA)
Lv, Wei& Shen, Yamei& Yang, Hengli& Yang, Rui& Cai, Wenbin& Zhang, Jian…[et al.]. A Novel Bimodal Imaging Agent Targeting HER2 Molecule of Breast Cancer. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192735
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192735