Serum and Ectopic Endometrium from Women with Endometriosis Modulate Macrophage M1M2 Polarization via the Smad2Smad3 Pathway
Joint Authors
Nie, Mei-Fang
Xie, Qi
Wu, Ya-Hong
He, Hua
Zou, Lu-Jie
She, Xiao-Ling
Wu, Xian-Qing
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Objective.
This study investigated the alterations in macrophage polarization in patients with endometriosis as well as the underlying molecular mechanisms.
Methods.
Peritoneal washings, serum samples, and endometrial tissues were collected from endometriosis patients and control subjects.
Endometrial stromal cells (ESCs) were isolated from endometrial tissue, and conditioned medium was prepared by treating ESCs with or without various concentrations of interleukin- (IL-) 6, estrogen, or progestin.
The frequencies of CD86+ and CD163+ cells and expression levels of these markers as well as the cytokines IL-12 and IL-10 were measured in THP-1- (human monocytic leukemia cell) derived macrophages.
Results.
There was a decrease in the percentage of CD86+ macrophages in the peritoneal wash solution of patients with endometriosis.
Ectopic endometrial homogenates could promote M1 to M2 macrophage polarization in response to lipopolysaccharide (LPS), as evidenced by the increased percentage of CD163+ macrophages and increased IL-10 expression as well as a decreased percentage of CD86+ cells and lower IL-12 expression.
In contrast, addition of serum from women with endometriosis to THP-1 cells resulted in the polarization of macrophages towards both M1 and M2 phenotypes.
Upregulation of Smad2/Smad3 in macrophages upon exposure to eutopic and ectopic endometrial homogenates as well as serum of women with endometriosis was observed, and blockage of Smad2/Smad3 with their inhibitor SB431542 could reverse the macrophage polarization from M1 to M2.
Conditioned medium induced by IL-6, but neither estrogen nor progestin, could facilitate M2 polarization.
Neutralization of IL-6 diminished macrophage M2 polarization in endometriosis.
Conclusion.
This study provides detailed evidence supporting alterations in M1 to M2 macrophage polarization that may contribute to the initiation as well as progression of endometriosis.
American Psychological Association (APA)
Nie, Mei-Fang& Xie, Qi& Wu, Ya-Hong& He, Hua& Zou, Lu-Jie& She, Xiao-Ling…[et al.]. 2018. Serum and Ectopic Endometrium from Women with Endometriosis Modulate Macrophage M1M2 Polarization via the Smad2Smad3 Pathway. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1192753
Modern Language Association (MLA)
Nie, Mei-Fang…[et al.]. Serum and Ectopic Endometrium from Women with Endometriosis Modulate Macrophage M1M2 Polarization via the Smad2Smad3 Pathway. Journal of Immunology Research No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1192753
American Medical Association (AMA)
Nie, Mei-Fang& Xie, Qi& Wu, Ya-Hong& He, Hua& Zou, Lu-Jie& She, Xiao-Ling…[et al.]. Serum and Ectopic Endometrium from Women with Endometriosis Modulate Macrophage M1M2 Polarization via the Smad2Smad3 Pathway. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1192753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192753