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Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway
Joint Authors
Wang, Xiaohong
Guo, Jiwei
Han, Yong
Liu, Jian
Zhang, Yingzhe
Cheng, Kai
Guo, Qingqun
Liu, Song
Sun, Hongguang
Hua, Yitong
Zhang, Guoqiang
Xu, Shujian
Guo, Fengli
Yang, Zhenlin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Plasma cell mastitis (PCM) is one of the most frequently encountered inflammatory diseases of the nonlactating breast.
However, its pathogenesis has remained unknown.
Methods.
In this study, we observed the ultrastructure changes of PCM by a transmission electron microscope.
The transcriptome expression difference of exosomes was detected by RNA-Seq; then, we confirmed the key difference genes by western blot and immunohistochemistry.
Finally, we established the mouse PCM model by tissue homogenate injection to validate the role of exosomes on the progression of PCM.
Results.
The analysis of the exosomal transcriptome expression difference between PCM and normal mammary tissues using RNA-Seq showed the differential genes and enrichment pathways involved in the course of PCM.
The decreased HSP90AA1 and EEF2, excessive production of p-AKT, and p-mTOR were consistent with clinical specimens.
Inhibition of exosome secretion significantly inhibited inflammatory cell infiltration, and the mammary duct had maintained a better structure in the PCM mouse model.
Conclusion.
Our results revealed the role of exosomes acting as critical signal introduction facilitators in the progression of plasma cell mastitis and identified potential key genes in the regulation of this process.
These results will help to dissect the molecular mechanism of PCM and provide therapeutic targets.
American Psychological Association (APA)
Wang, Xiaohong& Han, Yong& Liu, Jian& Zhang, Yingzhe& Cheng, Kai& Guo, Jiwei…[et al.]. 2019. Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1192863
Modern Language Association (MLA)
Wang, Xiaohong…[et al.]. Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway. Mediators of Inflammation No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1192863
American Medical Association (AMA)
Wang, Xiaohong& Han, Yong& Liu, Jian& Zhang, Yingzhe& Cheng, Kai& Guo, Jiwei…[et al.]. Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1192863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192863