miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus

Abstract EN

MicroRNA (miRNA) has been widely suggested to play a vital role of in the pathogenesis of gestational diabetes mellitus (GDM).

We have previously demonstrated that miR-657 can regulate macrophage inflammatory response in GDM.

However, the role of miR-657 on M1/M2 macrophage polarization in GDM pathogenesis is not clear yet.

This study is aimed at elucidating this issue and identifying novel potential GDM therapeutic targets based on miRNA network.

miR-657 is found to be upregulated in placental macrophages demonstrated by real-time PCR, which can enhance macrophage proliferation and migration in vitro.

Luciferase reporter assay shows the evidence that FAM46C is a target of miR-657.

In addition, miR-657 can promote macrophage polarization toward the M1 phenotype by downregulating FAM46C in macrophages.

The present study strongly suggests miR-657 is involved in GDM pathogenesis by regulating macrophage proliferation, migration, and polarization via targeting FAM46C.

miR-657/FAM46C may serve as promising targets for GDM diagnosis and treatment.