Impaired Hippocampal Glutamate and Glutamine Metabolism in the dbdb Mouse Model of Type 2 Diabetes Mellitus
Joint Authors
Andersen, Jens Velde
Nissen, Jakob Dahl
Christensen, Sofie Kjellerup
Markussen, Kia Hjulmand
Waagepetersen, Helle Sønderby
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Type 2 diabetes mellitus (T2DM) is a risk factor for the development of Alzheimer’s disease, and changes in brain energy metabolism have been suggested as a causative mechanism.
The aim of this study was to investigate the cerebral metabolism of the important amino acids glutamate and glutamine in the db/db mouse model of T2DM.
Glutamate and glutamine are both substrates for mitochondrial oxidation, and oxygen consumption was assessed in isolated brain mitochondria by Seahorse XFe96 analysis.
In addition, acutely isolated cerebral cortical and hippocampal slices were incubated with [U-13C]glutamate and [U-13C]glutamine, and tissue extracts were analyzed by gas chromatography-mass spectrometry.
The oxygen consumption rate using glutamate and glutamine as substrates was not different in isolated cerebral mitochondria of db/db mice compared to controls.
Hippocampal slices of db/db mice exhibited significantly reduced 13C labeling in glutamate, glutamine, GABA, citrate, and aspartate from metabolism of [U-13C]glutamate.
Additionally, reduced 13C labeling were observed in GABA, citrate, and aspartate from [U-13C]glutamine metabolism in hippocampal slices of db/db mice when compared to controls.
None of these changes were observed in cerebral cortical slices.
The results suggest specific hippocampal impairments in glutamate and glutamine metabolism, without affecting mitochondrial oxidation of these substrates, in the db/db mouse.
American Psychological Association (APA)
Andersen, Jens Velde& Nissen, Jakob Dahl& Christensen, Sofie Kjellerup& Markussen, Kia Hjulmand& Waagepetersen, Helle Sønderby. 2017. Impaired Hippocampal Glutamate and Glutamine Metabolism in the dbdb Mouse Model of Type 2 Diabetes Mellitus. Neural Plasticity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1192901
Modern Language Association (MLA)
Andersen, Jens Velde…[et al.]. Impaired Hippocampal Glutamate and Glutamine Metabolism in the dbdb Mouse Model of Type 2 Diabetes Mellitus. Neural Plasticity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1192901
American Medical Association (AMA)
Andersen, Jens Velde& Nissen, Jakob Dahl& Christensen, Sofie Kjellerup& Markussen, Kia Hjulmand& Waagepetersen, Helle Sønderby. Impaired Hippocampal Glutamate and Glutamine Metabolism in the dbdb Mouse Model of Type 2 Diabetes Mellitus. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1192901
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192901