A Hypothalamic Leptin-Glutamate Interaction in the Regulation of Sympathetic Nerve Activity
Joint Authors
Zheng, Hong
Patel, Kaushik P.
Liu, Xuefei
Li, Yulong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Accumulated evidence indicates that obesity-induced type 2 diabetes (T2D) is associated with enhanced sympathetic activation.
The present study was conducted to investigate the role for leptin-glutamate signaling within the hypothalamus in regulating sympathetic nerve activity.
In anesthetized rats, microinjections of leptin (5 ng ~ 100 ng) into the arcuate nucleus (ARCN) and paraventricular nucleus (PVN) induced increases in renal sympathetic nerve activity (RSNA), blood pressure (BP), and heart rate (HR).
Prior microinjections of NMDA receptor antagonist AP5 (16 pmol) into the ARCN or PVN reduced leptin-induced increases in RSNA, BP, and HR in both ARCN and PVN.
Knockdown of a leptin receptor with siRNA inhibited NMDA-induced increases in RSNA, BP, and HR in the ARCN but not in the PVN.
Confocal calcium imaging in the neuronal NG108 and astrocytic C6 cells demonstrated that preincubation with leptin induced an increase in intracellular calcium green fluorescence when the cells were challenged with glutamate.
In high-fat diet and low-dose streptozotocin-induced T2D rats, we found that leptin receptor and NMDA NR1 receptor expressions in the ARCN and PVN were significantly increased.
In conclusion, these studies provide evidence that within the hypothalamic nuclei, leptin-glutamate signaling regulates the sympathetic activation.
This may contribute to the sympathoexcitation commonly observed in obesity-related T2D.
American Psychological Association (APA)
Zheng, Hong& Liu, Xuefei& Li, Yulong& Patel, Kaushik P.. 2017. A Hypothalamic Leptin-Glutamate Interaction in the Regulation of Sympathetic Nerve Activity. Neural Plasticity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1192905
Modern Language Association (MLA)
Zheng, Hong…[et al.]. A Hypothalamic Leptin-Glutamate Interaction in the Regulation of Sympathetic Nerve Activity. Neural Plasticity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1192905
American Medical Association (AMA)
Zheng, Hong& Liu, Xuefei& Li, Yulong& Patel, Kaushik P.. A Hypothalamic Leptin-Glutamate Interaction in the Regulation of Sympathetic Nerve Activity. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1192905
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192905