Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets
Joint Authors
Martínez-Peinado, Pascual
Pascual-García, Sandra
Roche, Enrique
Sempere-Ortells, José Miguel
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Mesenchymal stem cells (MSC) are a widely used population in cell therapy for their ability to differentiate into distinct tissues and more lately, for their immunomodulatory properties.
However, the use of heterogeneous populations could be responsible for the nondesired outcomes reflected in the literature.
Here, we analyse the different capacities of five one-cell-derived MSC clones to exert their immunomodulation ex vivo.
We assessed proliferation assays in cocultures of MSC clones and purified cluster of differentiation (CD)3+, CD4+, or CD8+ lymphocytes; analysed the regulatory T (Treg) cells fold change rate; determined the effects on viability of peripheral blood mononuclear cells (PBMC); and also measured the coculture cytokine profiles (Th1/Th2).
Conditioned media (CM) of different clones were also used to perform both proliferation assays and to analyse Treg fold change.
The five clones analysed in this work were able to generate heterogeneous environments.
Different clones inhibited proliferation of CD3+ and CD4+ lymphocytes, with different intensities.
Surprisingly, all clones promoted proliferation of CD8+ lymphocytes.
Different MSC clones and their CM were able to increase the number of Treg with different intensities.
Finally, different clones also promoted different effects on the viability of PBMC treated with ultraviolet light.
Considering all these data together, it seems that different clones, even from the same donor, can promote a wide spectrum of responses from anti-inflammatory to proinflammatory character.
This fact may be important to standardise the design of personalized cell therapy protocols, thus diminishing the aforementioned undesired outcomes existing nowadays in this type of therapies.
American Psychological Association (APA)
Martínez-Peinado, Pascual& Pascual-García, Sandra& Roche, Enrique& Sempere-Ortells, José Miguel. 2018. Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1192921
Modern Language Association (MLA)
Martínez-Peinado, Pascual…[et al.]. Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets. Journal of Immunology Research No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1192921
American Medical Association (AMA)
Martínez-Peinado, Pascual& Pascual-García, Sandra& Roche, Enrique& Sempere-Ortells, José Miguel. Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1192921
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192921