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MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis
Joint Authors
Méndez-Flores, Silvia
Hernández-Molina, Gabriela
Ramírez-Martinez, Gustavo
Regino-Zamarripa, Nora E.
Ortiz-Quintero, Blanca
Jiménez-Alvarez, Luis
Cruz-Lagunas, Alfredo
Furuzawa-Carballeda, Janette
Zuñiga, Joaquin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-26, 26 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-30
Country of Publication
Egypt
No. of Pages
26
Main Subjects
Abstract EN
Background.
The role of miRNAs in the pathogenesis of cutaneous lupus has not been studied.
Objective.
It was to assess the levels of a selected panel of circulating miRNAs that could be involved in the regulation of the immune response, inflammation, and fibrosis in cutaneous lupus.
Methods.
It was a cross-sectional study.
We included 22 patients with subacute (SCLE) and 20 with discoid (DLE) lesions, and 19 healthy donors (HD).
qRT-PCR for miRNA analysis, flow cytometry in peripheral blood, and skin immunohistochemistry were performed to determine the distribution of CD4 T cells and regulatory cells and their correlation with circulating miRNAs.
Results.
miR-150, miR-1246, miR-21, miR-23b, and miR-146 levels were downregulated in SCLE vs.
HD.
miR-150, miR-1246, and miR-21 levels were downregulated in DLE vs.
HD.
Peripheral CD4+/CD25-/IL-4+ cells and CD4+/CD25hi/Foxp3+ were negatively associated with miR-23b, and CD4+/CD25-/IFN-γ+ with miR-1246 in SCLE, whereas CD123+/CD196+/IDO+ cells were positively associated with miR-150 in DLE.
In the tissue, CD4+/IL-4+ and CD20+/IL-10+ cells were positively associated with miR-21 and CD4+/IFN-γ+ with miR-31 in SCLE, whereas CD4+/IL-4+ cells were positively associated with miR-150, and CD20+/IL-10+ cells with miR-1246 and miR-146a in DLE.
In the SCLE, lower miR-150 levels were correlated with higher CLASI scores.
The KEGG pathway enrichment analysis revealed that cell cycle regulation pathways, p53, TGF-β, thyroid hormone, and cancer signaling pathways were shared between miR-21, miR-31, miR-23b, miR-146a, miR-1246, and miR-150.
Conclusions.
A downregulation of miR-150, miR-1246, and miR-21 in both CLE varieties vs.
HD was determined.
American Psychological Association (APA)
Méndez-Flores, Silvia& Furuzawa-Carballeda, Janette& Hernández-Molina, Gabriela& Ramírez-Martinez, Gustavo& Regino-Zamarripa, Nora E.& Ortiz-Quintero, Blanca…[et al.]. 2019. MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-26.
https://search.emarefa.net/detail/BIM-1192928
Modern Language Association (MLA)
Méndez-Flores, Silvia…[et al.]. MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis. Mediators of Inflammation No. 2019 (2019), pp.1-26.
https://search.emarefa.net/detail/BIM-1192928
American Medical Association (AMA)
Méndez-Flores, Silvia& Furuzawa-Carballeda, Janette& Hernández-Molina, Gabriela& Ramírez-Martinez, Gustavo& Regino-Zamarripa, Nora E.& Ortiz-Quintero, Blanca…[et al.]. MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-26.
https://search.emarefa.net/detail/BIM-1192928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192928