PG102 Upregulates IL-37 through p38, ERK, and Smad3 Pathways in HaCaT Keratinocytes
Joint Authors
Kim, Hyun-keun
Lim, Seonung
Lee, Wonwoo
Kim, Sunyoung
Bae, Min-Jung
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
IL-37 is an immunomodulatory cytokine that suppresses inflammation in various cell types and disease models.
However, its role in keratinocytes has not been clearly understood, and there has been no report on the agents that can increase the expression of IL-37 in keratinocytes.
In this study, we investigated the effects of silencing IL37 in HaCaT keratinocytes and the molecular mechanisms involved in the upregulation of IL-37 by PG102, a water-soluble extract from Actinidia arguta.
It was found that knockdown of IL37 resulted in the augmented expression of antimicrobial peptides (AMPs) in response to cytokine stimulation.
PG102 increased the expression of IL-37 at both mRNA and protein levels presumably by enhancing the phosphorylation of Smad3, ERK, and p38.
Indeed, when cells were treated with specific inhibitors for these signaling molecules, the expression level of IL-37 was reduced.
PG102 also promoted colocalization of phospho-Smad3 and IL-37.
Our results suggest that IL-37 inhibits the expression of AMPs and that PG102 upregulates IL-37 through p38, ERK, and Smad3 pathways in HaCaT cells.
American Psychological Association (APA)
Kim, Hyun-keun& Lim, Seonung& Bae, Min-Jung& Lee, Wonwoo& Kim, Sunyoung. 2019. PG102 Upregulates IL-37 through p38, ERK, and Smad3 Pathways in HaCaT Keratinocytes. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1193000
Modern Language Association (MLA)
Kim, Hyun-keun…[et al.]. PG102 Upregulates IL-37 through p38, ERK, and Smad3 Pathways in HaCaT Keratinocytes. Mediators of Inflammation No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1193000
American Medical Association (AMA)
Kim, Hyun-keun& Lim, Seonung& Bae, Min-Jung& Lee, Wonwoo& Kim, Sunyoung. PG102 Upregulates IL-37 through p38, ERK, and Smad3 Pathways in HaCaT Keratinocytes. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1193000
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193000