Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice
Joint Authors
Wang, Zhugang
Yang, Hua
Zhang, Mengjie
Shi, Jiahao
Zhou, Yunhe
Wan, Zhipeng
Wang, Yicheng
Wan, Yinghan
Li, Jun
Fei, Jian
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Several studies have associated reduced expression of synaptosomal-associated protein of 25 kDa (SNAP-25) with schizophrenia, yet little is known about its role in the illness.
In this paper, a forebrain glutamatergic neuron-specific SNAP-25 knockout mouse model was constructed and studied to explore the possible pathogenetic role of SNAP-25 in schizophrenia.
We showed that SNAP-25 conditional knockout (cKO) mice exhibited typical schizophrenia-like phenotype.
A significantly elevated extracellular glutamate level was detected in the cerebral cortex of the mouse model.
Compared with Ctrls, SNAP-25 was dramatically reduced by about 60% both in cytoplasm and in membrane fractions of cerebral cortex of cKOs, while the other two core members of SNARE complex: Syntaxin-1 (increased ~80%) and Vamp2 (increased ~96%) were significantly increased in cell membrane part.
Riluzole, a glutamate release inhibitor, significantly attenuated the locomotor hyperactivity deficits in cKO mice.
Our findings provide in vivo functional evidence showing a critical role of SNAP-25 dysfunction on synaptic transmission, which contributes to the developmental of schizophrenia.
It is suggested that a SNAP-25 cKO mouse, a valuable model for schizophrenia, could address questions regarding presynaptic alterations that contribute to the etiopathophysiology of SZ and help to consummate the pre- and postsynaptic glutamatergic pathogenesis of the illness.
American Psychological Association (APA)
Yang, Hua& Zhang, Mengjie& Shi, Jiahao& Zhou, Yunhe& Wan, Zhipeng& Wang, Yicheng…[et al.]. 2017. Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice. Neural Plasticity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1193014
Modern Language Association (MLA)
Yang, Hua…[et al.]. Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice. Neural Plasticity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1193014
American Medical Association (AMA)
Yang, Hua& Zhang, Mengjie& Shi, Jiahao& Zhou, Yunhe& Wan, Zhipeng& Wang, Yicheng…[et al.]. Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1193014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193014