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Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3KAKT-eNOS Signalling in HUVECs
Joint Authors
Tang, Qi-Zhu
Deng, Wei
Zhou, Heng
Duan, Ming-Xia
Wu, Qing-Qing
Liu, Chen
Xiao, Yang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-07
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Andrographolide (Andr) is a major component isolated from the plant Andrographis paniculata.
Inflammation, apoptosis, and impaired angiogenesis are implicated in the pathogenesis of high glucose (HG)-induced injury of vascular endotheliocytes.
Our study is aimed at evaluating the effect of Andr on HG-induced HUVEC injury and the underlying mechanism.
HUVECs were exposed to HG levels (33 mM) and treated with Andr (0, 12.5, 25, and 50 μM).
Western blot analysis, real-time PCR, immunofluorescence staining, the scratch test, and the tube formation assay were performed to assess the effects of Andr.
We discovered that Andr inhibited the inflammatory response (IL-1β, IL-6, and TNFα), decreased the apoptosis ratio and cell migration, and promoted tube formation in response to HG stimulation.
Andr ameliorated the levels of phosphorylated PI3K (p-PI3K), phosphorylated AKT (p-AKT), and phosphorylated eNOS (p-eNOS).
The expression of vascular endothelial growth factor (VEGF) protein, a vital factor in angiogenesis, was improved by Andr treatment under HG stimulation.
LY294002 is a blocker of PI3K, MK-2206 2HCI (MK-2206) is a highly selective AKT inhibitor, and L-NAME is a suppressor of eNOS, all of which significantly reduce Andr-mediated protective effects in vitro.
Hence, Andr may be involved in regulating HG-induced injury by activating PI3K/AKT-eNOS signalling in HUVECs.
American Psychological Association (APA)
Duan, Ming-Xia& Zhou, Heng& Wu, Qing-Qing& Liu, Chen& Xiao, Yang& Deng, Wei…[et al.]. 2019. Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3KAKT-eNOS Signalling in HUVECs. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1193022
Modern Language Association (MLA)
Duan, Ming-Xia…[et al.]. Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3KAKT-eNOS Signalling in HUVECs. Mediators of Inflammation No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1193022
American Medical Association (AMA)
Duan, Ming-Xia& Zhou, Heng& Wu, Qing-Qing& Liu, Chen& Xiao, Yang& Deng, Wei…[et al.]. Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3KAKT-eNOS Signalling in HUVECs. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1193022
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193022