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Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase
Joint Authors
Tonna, Noemi
Biggi, Silvia
Buccarello, Lucia
Sclip, Alessandra
Lippiello, Pellegrino
Rumio, Cristiano
Di Marino, Daniele
Miniaci, Maria Concetta
Borsello, Tiziana
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The c-Jun N-terminal kinase (JNK) is part of a stress signalling pathway strongly activated by NMDA-stimulation and involved in synaptic plasticity.
Many studies have been focused on the post-synaptic mechanism of JNK action, and less is known about JNK presynaptic localization and its physiological role at this site.
Here we examined whether JNK is present at the presynaptic site and its activity after presynaptic NMDA receptors stimulation.
By using N-SIM Structured Super Resolution Microscopy as well as biochemical approaches, we demonstrated that presynaptic fractions contained significant amount of JNK protein and its activated form.
By means of modelling design, we found that JNK, via the JBD domain, acts as a physiological effector on T-SNARE proteins; then using biochemical approaches we demonstrated the interaction between Syntaxin-1-JNK, Syntaxin-2-JNK, and Snap25-JNK.
In addition, taking advance of the specific JNK inhibitor peptide, D-JNKI1, we defined JNK action on the SNARE complex formation.
Finally, electrophysiological recordings confirmed the role of JNK in the presynaptic modulation of vesicle release.
These data suggest that JNK-dependent phosphorylation of T-SNARE proteins may have an important functional role in synaptic plasticity.
American Psychological Association (APA)
Biggi, Silvia& Buccarello, Lucia& Sclip, Alessandra& Lippiello, Pellegrino& Tonna, Noemi& Rumio, Cristiano…[et al.]. 2017. Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase. Neural Plasticity،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1193140
Modern Language Association (MLA)
Biggi, Silvia…[et al.]. Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase. Neural Plasticity No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1193140
American Medical Association (AMA)
Biggi, Silvia& Buccarello, Lucia& Sclip, Alessandra& Lippiello, Pellegrino& Tonna, Noemi& Rumio, Cristiano…[et al.]. Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1193140
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193140