Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells
Joint Authors
Huang, Chunji
He, Binfeng
Hu, Mingdong
Wang, Guansong
Chen, Huaping
Yuan, Ming
Xu, Zhi
Li, Mingchun
Zhang, Chun
Gao, Zhan
Zhang, Mingzhou
Xu, Jiancheng
Qian, Hang
You, Jiegen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Rab26 GTPase modulates the trafficking of cell surface receptors, such as G protein-coupled receptors including α2-adrenergic receptors in some cell types.
However, the effect of Rab26 on β2-adrenergic receptor (β2-AR) trafficking or/and Toll-like receptor 4 (TLR4) expression in human pulmonary microvascular endothelial cells (HPMECs) is still unclear.
Here, we investigated the role of Rab26 in regulating the expression of β2-ARs and TLR4 in HPMECs and the effect of these receptors’ imbalance on endothelial cell barrier function.
The results showed that there was unbalance expression in these receptors, where β2-AR expression was remarkably reduced, and TLR4 was increased on the cell membrane after lipopolysaccharide (LPS) treatment.
Furthermore, we found that Rab26 overexpression not only upregulated β2-ARs but also downregulated TLR4 expression on the cell membrane.
Subsequently, the TLR4-related inflammatory response was greatly attenuated, and the hyperpermeability of HPMECs also was partially relived.
Taken together, these data suggest that basal Rab26 maintains the balance between β2-ARs and TLR4 on the cell surface, and it might be a potential therapeutic target for diseases involving endothelial barrier dysfunction.
American Psychological Association (APA)
Chen, Huaping& Yuan, Ming& Huang, Chunji& Xu, Zhi& Li, Mingchun& Zhang, Chun…[et al.]. 2019. Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1193285
Modern Language Association (MLA)
Chen, Huaping…[et al.]. Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells. Mediators of Inflammation No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1193285
American Medical Association (AMA)
Chen, Huaping& Yuan, Ming& Huang, Chunji& Xu, Zhi& Li, Mingchun& Zhang, Chun…[et al.]. Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1193285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193285