Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma
Joint Authors
Einspanier, Ralf
Barton, Ann Kristin
Shety, Tarek
Gehlen, Heidrun
Klier, John
Geis, Sabine
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG-ODN) normalizes clinical and cytologic parameters in severe equine asthma.
We hypothesized that CpG-ODN inhalation also reduces the misbalance of elastinolytic activity in asthmatic horses.
Methods.
Twenty asthmatic horses diagnosed by clinical examinations using a scoring system were included.
All horses inhaled CpG-ODNs for 14 days in 2-day intervals.
Matrix metalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinase (TIMP-1/-2) concentrations were measured in tracheal aspirates using equine sandwich ELISAs before and 2 and 6 weeks after CpG-ODN inhalation.
Results.
MMP and TIMP concentrations correlated with the results of clinical scoring in all stages of equine asthma.
Inhalation therapy led to significant reductions in clinical scores.
MMP-2, MMP-9, and TIMP-2 concentrations were significantly reduced immediately, and all MMP and TIMP concentrations 6 weeks after therapy.
Discussion.
In equine asthma, overexpression of MMPs contributes to pathological tissue destruction, while TIMPs counteract MMPs with overexpression leading to fibrosis formation.
The results of this study show that CpG-ODN inhalation may be an effective therapy to address a misbalance in equine asthma.
Conclusions.
Misbalance of elastinolytic activity seems to improve by CpG-ODN inhalation for at least 6 weeks posttherapy, which may reduce the remodeling of the extracellular matrix.
Further studies should evaluate this effect in comparison to glucocorticoid inhalation therapy.
Significance.
CpG-ODN inhalation may be an effective therapy in the prevention of pulmonary fibrosis formation in equine asthma.
American Psychological Association (APA)
Barton, Ann Kristin& Shety, Tarek& Klier, John& Geis, Sabine& Einspanier, Ralf& Gehlen, Heidrun. 2019. Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1193326
Modern Language Association (MLA)
Barton, Ann Kristin…[et al.]. Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma. Mediators of Inflammation No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1193326
American Medical Association (AMA)
Barton, Ann Kristin& Shety, Tarek& Klier, John& Geis, Sabine& Einspanier, Ralf& Gehlen, Heidrun. Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1193326
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193326