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Root-Securing and Brain-Fortifying Liquid Upregulates Caveolin-1 in Cell Model with Alzheimer’s Disease through Inhibiting Tau Phosphorylation
Joint Authors
Chen, Ning
Yuan, Depei
Zeng, Chuhua
Chen, Qianfeng
Wang, Fengjie
Yuan, Lin
Zhu, Yaoqian
Shu, Ziyang
Source
Neurology Research International
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In order to explore the effect of root-securing and brain-fortifying Liquid- (RSBFL-) mediated caveolin-1 (CAV-1) on phosphorylation of Tau protein and to uncover underlying mechanisms of RSBFL for the prevention and treatment of Alzheimer’s disease (AD), hippocampal neurons isolated from neonatal SD rats and cultured in DMEM-F12 medium were induced by exogenous Aβ1–42 to establish a cell model with AD.
Meanwhile, pEGFP-C1-CAV1 and CAV1-shRNA plasmids were transfected into hippocampal neurons for CAV-1 overexpression and silence, respectively.
The serum containing RSBFL was prepared for the intervention of AD model cells.
The expression of CAV-1, GSK-3β, and p-Tau in normal hippocampal neurons and AD model cells in the presence of serum containing RSBFL was evaluated.
The model hippocampal neurons with AD induced by Aβ1–42 revealed an obvious CAV-1 inhibition, enhanced GSK-3β activity, and abnormal Tau phosphorylation.
In contrast, the treatment with serum containing RSBFL could upregulate CAV-1 in AD hippocampal neurons (P<0.05) with improved p-GSK-3βSer9 and reduced p-GSK-3βTyr216 (P<0.01), as well as suppressed abnormal phosphorylation of Tau protein.
Therefore, RSBFL has an excellent protective effect on hippocampal neurons through increasing CAV-1 expression, inhibiting GSK-3β activity, and reducing excessive abnormal phosphorylation of Tau protein.
American Psychological Association (APA)
Yuan, Depei& Zeng, Chuhua& Chen, Qianfeng& Wang, Fengjie& Yuan, Lin& Zhu, Yaoqian…[et al.]. 2017. Root-Securing and Brain-Fortifying Liquid Upregulates Caveolin-1 in Cell Model with Alzheimer’s Disease through Inhibiting Tau Phosphorylation. Neurology Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1193416
Modern Language Association (MLA)
Yuan, Depei…[et al.]. Root-Securing and Brain-Fortifying Liquid Upregulates Caveolin-1 in Cell Model with Alzheimer’s Disease through Inhibiting Tau Phosphorylation. Neurology Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1193416
American Medical Association (AMA)
Yuan, Depei& Zeng, Chuhua& Chen, Qianfeng& Wang, Fengjie& Yuan, Lin& Zhu, Yaoqian…[et al.]. Root-Securing and Brain-Fortifying Liquid Upregulates Caveolin-1 in Cell Model with Alzheimer’s Disease through Inhibiting Tau Phosphorylation. Neurology Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1193416
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193416