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Paneth Cell Ablation Aggravates Pancreatic and Intestinal Injuries in a Rat Model of Acute Necrotizing Pancreatitis after Normal and High-Fat Diet
Joint Authors
Zeng, Yue
Guo, Yuecheng
Huang, Chunlan
Liu, Liyan
Fu, Xinyuan
Lu, Yingying
Zheng, Junyuan
Mei, Qixiang
Huang, Zehua
Fan, Junjie
Lu, Lungen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-11
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
We previously reported that acute necrotizing pancreatitis (ANP) after normal or high-fat diet is associated with a decreased number of Paneth cells in ileal crypts.
Here, we ablated Paneth cells in a rat model of ANP after normal and high-fat diet to investigate the effects on disease symptoms.
Adult male Sprague-Dawley rats received standard rat chow or a high-fat diet for 2 weeks, after which they were treated with dithizone to deplete Paneth cells.
Six hours later, ANP was established by retrograde injection of sodium taurocholate into the biliopancreatic duct.
Rats were sacrificed at 6, 12, and 24 h for assessment.
We found dithizone aggravated ANP-associated pathological injuries to the pancreas and ileum in rats on high-fat or standard diets.
Lysozyme expression in ileal crypts was decreased, while serum inflammatory cytokines (TNFα, IL-1β, and IL-17A) and intestinal permeability (serum DAO activity and D-lactate) were increased.
Expression of tight junction proteins (claudin-1, zo-1, and occludin) was decreased.
Using high-throughput 16S rRNA sequencing, we found dithizone reduced microbiota diversity and altered microbiota composition in rats on high-fat or standard diets.
Dithizone decreased fecal short-chain fatty acids (SCFAs) in rats on high-fat or standard diets.
Changes in intestinal microbiota correlated significantly with SCFAs, lysozyme, DAO activity, D-lactate, inflammatory cytokines, and pathological injury to the pancreas and ileum in rats on high-fat or standard diets.
In conclusion, ablation of Paneth cells exacerbates pancreatic and intestinal injuries in ANP after normal and high-fat diet.
These symptoms may be related to changes in the intestinal microbiota.
American Psychological Association (APA)
Guo, Yuecheng& Huang, Chunlan& Liu, Liyan& Fu, Xinyuan& Lu, Yingying& Zheng, Junyuan…[et al.]. 2019. Paneth Cell Ablation Aggravates Pancreatic and Intestinal Injuries in a Rat Model of Acute Necrotizing Pancreatitis after Normal and High-Fat Diet. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1193494
Modern Language Association (MLA)
Guo, Yuecheng…[et al.]. Paneth Cell Ablation Aggravates Pancreatic and Intestinal Injuries in a Rat Model of Acute Necrotizing Pancreatitis after Normal and High-Fat Diet. Mediators of Inflammation No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1193494
American Medical Association (AMA)
Guo, Yuecheng& Huang, Chunlan& Liu, Liyan& Fu, Xinyuan& Lu, Yingying& Zheng, Junyuan…[et al.]. Paneth Cell Ablation Aggravates Pancreatic and Intestinal Injuries in a Rat Model of Acute Necrotizing Pancreatitis after Normal and High-Fat Diet. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1193494
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193494